Defining genetic and chemical diversity in wheat grain by 1H-NMR spectroscopy of polar metabolites

A - Papers appearing in refereed journals

Shewry, P. R., Corol, D. I., Jones, H. D., Beale, M. H. and Ward, J. L. 2017. Defining genetic and chemical diversity in wheat grain by 1H-NMR spectroscopy of polar metabolites. Molecular Nutrition & Food Research. 61 (7), p. 1600807.

AuthorsShewry, P. R., Corol, D. I., Jones, H. D., Beale, M. H. and Ward, J. L.
Abstract

Scope
The application of high‐throughput 1H nuclear magnetic resonance (1H‐NMR) of unpurified extracts to determine genetic diversity and the contents of polar components in grain of wheat.

Methods and results
Milled whole wheat grain was extracted with 80:20 D2O:CD3OD containing 0.05% d4–trimethylsilylpropionate. 1H‐NMR spectra were acquired under automation at 300°K using an Avance Spectrometer operating at 600.0528 MHz. Regions for individual metabolites were identified by comparison to a library of known standards run under identical conditions. The individual 1H‐NMR peaks or levels of known metabolites were then compared by Principal Component Analysis using SIMCA‐P software.

Conclusions
High‐throughput 1H‐NMR is an excellent tool to compare the extent of genetic diversity within and between wheat species, and to quantify specific components (including glycine betaine, choline, and asparagine) in individual genotypes. It can also be used to monitor changes in composition related to environmental factors and to support comparisons of the substantial equivalence of transgenic lines.

KeywordsGrain composition; Metabolomics; NMR Spectroscopy; Transgenic; Wheat
Year of Publication2017
JournalMolecular Nutrition & Food Research
Journal citation61 (7), p. 1600807
Digital Object Identifier (DOI)doi:10.1002/mnfr.201600807
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
European Union
Funder project or codeQuality (WP2) [DFW-ISPG]
A new generation of insect resistant GM crops: transgenic wheat synthesising the aphid alarm signal
Project HEALTHGRAIN
Publisher's version
Output statusPublished
Publication dates
Online13 Jan 2017
Publication process dates
Accepted10 Jan 2017
Copyright licenseCC BY
PublisherWiley
ISSN1613-4125

Permalink - https://repository.rothamsted.ac.uk/item/8v471/defining-genetic-and-chemical-diversity-in-wheat-grain-by-1h-nmr-spectroscopy-of-polar-metabolites

28 total views
2 total downloads
7 views this month
0 downloads this month
Download files as zip