Chromosomal assignment of genes in barley using telosomic wheat-barley addition lines

A - Papers appearing in refereed journals

Cannell, M., Karp, A., Isaac, P. G. and Shewry, P. R. 1992. Chromosomal assignment of genes in barley using telosomic wheat-barley addition lines. Genome. 35 (1), pp. 17-23.

AuthorsCannell, M., Karp, A., Isaac, P. G. and Shewry, P. R.
Abstract

Characterized DNA clones have been used as probes in Southern blotting and hybridization analysis of genomic DNA from a set of wheat-barley telosomic addition lines to assign genes to chromosome arms in barley (Hordeum vulgare). The lines used included a newly available addition line with the short arm of chromosome 5. Addition lines with the long arm or the whole of chromosome 5 of H. vulgare are not available, so addition lines with chromosomes 5 and 5S of a related wild species, Hordeum chilense, were also used. A total of 23 characterized cDNAs and two characterized genomic clones were mapped to chromosome arms in this way.

KeywordsGene Mapping; telosomic additions; cdna clones; wheat-barley; restriction fragment length polymorphisms; fragment-length-polymorphism; amylase subtilisin inhibitor; cdna clone; hordeum-vulgare; nucleotide-sequence; messenger-rna; molecular analysis; expression; aleurone; Identification
Year of Publication1992
JournalGenome
Journal citation35 (1), pp. 17-23
Digital Object Identifier (DOI)doi:10.1139/g92-003
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
ISSN08312796

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