Chromosomal assignment of genes in barley using telosomic wheat-barley addition lines

A - Papers appearing in refereed journals

Cannell, M., Karp, A., Isaac, P. G. and Shewry, P. R. 1992. Chromosomal assignment of genes in barley using telosomic wheat-barley addition lines. Genome. 35 (1), pp. 17-23. https://doi.org/10.1139/g92-003

AuthorsCannell, M., Karp, A., Isaac, P. G. and Shewry, P. R.
Abstract

Characterized DNA clones have been used as probes in Southern blotting and hybridization analysis of genomic DNA from a set of wheat-barley telosomic addition lines to assign genes to chromosome arms in barley (Hordeum vulgare). The lines used included a newly available addition line with the short arm of chromosome 5. Addition lines with the long arm or the whole of chromosome 5 of H. vulgare are not available, so addition lines with chromosomes 5 and 5S of a related wild species, Hordeum chilense, were also used. A total of 23 characterized cDNAs and two characterized genomic clones were mapped to chromosome arms in this way.

KeywordsGene Mapping; telosomic additions; cdna clones; wheat-barley; restriction fragment length polymorphisms; fragment-length-polymorphism; amylase subtilisin inhibitor; cdna clone; hordeum-vulgare; nucleotide-sequence; messenger-rna; molecular analysis; expression; aleurone; Identification
Year of Publication1992
JournalGenome
Journal citation35 (1), pp. 17-23
Digital Object Identifier (DOI)https://doi.org/10.1139/g92-003
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
ISSN08312796

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