Molecular analysis of a mutation conferring the high-lysine phenotype on the grain of barley (hordeum vulgare)

A - Papers appearing in refereed journals

Kreis, M., Shewry, P. R., Forde, B. G., Rahman, S. and Miflin, B. J. 1983. Molecular analysis of a mutation conferring the high-lysine phenotype on the grain of barley (hordeum vulgare). Cell. 34 (1), pp. 161-167.

AuthorsKreis, M., Shewry, P. R., Forde, B. G., Rahman, S. and Miflin, B. J.
Abstract

We have analyzed the molecular nature of the Rise 56 mutation that occurs in barley. This mutation results in a depression of hordein accumulation in the grain and consequently in a higher overall lysine content. In particular, the amount of B hordein, which is encoded by the complex locus Hor-2, is decreased by about 75% because of the absence of the major components. The synthesis of certain minor polypeptides, with properties similar to the major B hordeins, remains unaffected. Analysis of endosperm RNA, by in vitro translation and hybridization to various cloned cDNAs derived from hordein mRNA, shows that mRNA for the major B hordeins is not present in the endosperm. Hybridization of a B hordein cDNA clone to gel-fractionated restriction digests of mutant and wild-type DNA indicates that at least 85 kb of DNA has been deleted from the Hor-2 locus in the high-lysine mutant.

Year of Publication1983
JournalCell
Journal citation34 (1), pp. 161-167
Digital Object Identifier (DOI)doi:10.1016/0092-8674(83)90146-0
Open accessPublished as non-open access
Output statusPublished
Publication dates
Online29 Apr 2004
Print01 Aug 1983
Copyright licensePublisher copyright
PublisherCell Press
ISSN0092-8674

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