Differential expression of two barley SNF1-related protein-kinase genes

A - Papers appearing in refereed journals

Hannappel, U., Vicentecarbajosa, J., Barker, J. H. A., Shewry, P. R. and Halford, N. G. 1995. Differential expression of two barley SNF1-related protein-kinase genes. Plant Molecular Biology. 27 (6), pp. 1235-1240.

AuthorsHannappel, U., Vicentecarbajosa, J., Barker, J. H. A., Shewry, P. R. and Halford, N. G.
Abstract

We have amplified and cloned DNA sequences derived from a gene encoding a SNF1 (sucrose-nonfermenting 1)-related protein kinase which differs from that previously reported from barley. Northern blot and polymerase chain reaction (PCR) analysis of RNA populations, using specific probes and oligonucleotide primers, indicated that the two SNF1-related genes are differentially regulated. One is expressed in all tissues, whereas the other is expressed at high levels in the seed endosperm and aleurone, but at levels undetectable by northern blot analysis in other tissues. Comparisons with other plant SNF1-related protein kinase genes suggest that the form which is expressed at greatly enhanced levels in the seed is less similar to the other plant homologues which have been reported and may be unique to cereals.

KeywordsGene Expression; gene family; higher plants; Hordeum Vulgare; metabolic; Regulation; acetyl-coa carboxylase; saccharomyces-cerevisiae; glucose repression; snf4 protein; Yeast; Metabolism; Phosphorylation; Identification; family; sites
Year of Publication1995
JournalPlant Molecular Biology
Journal citation27 (6), pp. 1235-1240
Digital Object Identifier (DOI)doi:10.1007/bf00020898
Output statusPublished
Publication process dates
Accepted17 Feb 2015
Copyright licenseCC BY
ISSN01674412

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