An homogeneous radioimmunoassay for abscisic acid using a scintillation proximity assay technique

A - Papers appearing in refereed journals

Whitford, P. N. and Croker, S. J. 1991. An homogeneous radioimmunoassay for abscisic acid using a scintillation proximity assay technique. Phytochemical Analysis. 2 (3), pp. 134-136. https://doi.org/10.1002/pca.2800020309

AuthorsWhitford, P. N. and Croker, S. J.
Abstract

An homogeneous radioimmunoassay (RIA) for the plant growth regulator abscisic acid (ABA) has been developed by modifying an established heterogeneous assay. The introduction of a scintillation proximity reagent has
enabled the production of a one‐step RIA in which radioactivity associated with antibody‐bound ABA can be counted in the presence of the unbound radiolabelled ABA and without the addition of a liquid scintillation cocktail. The assay has been validated by comparing the results with those obtained by combined gas chromatography/mass spectrometry and the precision of each method determined. The homogeneous RIA has a working range of 0.165–13.2 ng ABA and has been used to determine ABA concentration in extracts of leaves of tomato, Lycopersicon esculentum Mill. cv. Ailsa Craig (wild type) and three wilty mutants, notabilis, flacca and sitiens.

KeywordsRadioimmunoassay; abscisic acid ; scintillation proximity assay
Year of Publication1991
JournalPhytochemical Analysis
Journal citation2 (3), pp. 134-136
Digital Object Identifier (DOI)https://doi.org/10.1002/pca.2800020309
Open accessPublished as non-open access
Publication dates
PrintJul 1991
PublisherWiley
Copyright licensePublisher copyright
ISSN0958-0344

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