Soil Characteristics Overwhelm Cultivar Effects on the Structure and Assembly of Root-Associated Microbiomes of Modern Maize

A - Papers appearing in refereed journals

Chen, L., Zhang, J., Redmile-Gordon, M. A., Nie, G. and Wang, Q. 2019. Soil Characteristics Overwhelm Cultivar Effects on the Structure and Assembly of Root-Associated Microbiomes of Modern Maize. Pedosphere. 29 (3), pp. 360-373.

AuthorsChen, L., Zhang, J., Redmile-Gordon, M. A., Nie, G. and Wang, Q.
Abstract

Modern breeding primarily targets crop yield traits and is likely to influence root-associated microbiomes, which play significant roles in plant growth and health. The relative importance of soil and cultivar factors in shaping root-associated microbiomes of modern maize (Zea mays L.) remains uncertain. We conducted a pot experiment in a controlled environment using three soils (Mollisol, Inceptisol, and Ultisol) and four contrasting cultivars, Denghai 605, Nonghua 816, Qiaoyu 8, and Zhengdan 958, which are widely planted in China. We used 16S rRNA gene amplicon sequencing to characterize the bacterial communities in the bulk soil, rhizosphere, and endosphere. Our results showed that the four cultivars had different shoot biomass and root exudate total organic carbon and organic acid contents. The microbiomes in the bulk soil, rhizosphere, and endosphere were different. We observed apparent community divergence between soils rather than cultivars, within which edaphic factors substantially contributed to microbiome variation. Moreover, permutational multivariate analysis of variance corroborated significant contributions of soil type but not cultivar on the root-associated microbiome structure. Differential abundance analysis confirmed that each soil presented a distinct root microbiome, while network analysis indicated different co-occurrence patterns of the root microbiome among the three soils. The core root microbiome members are implicated in plant growth promotion and nutrient acquisition in the roots. In conclusion, root-associated microbiomes of modern maize are much more controlled by soil characteristics than by cultivar root exudation. Our study is anticipated to help improve breeding strategies through integrative interactions of soils, cultivars, and their associated microbiomes.

Keywordsamplicon sequencing; edaphic properties; endosphere; indigenous microbes; rhizosphere; root exudation
Year of Publication2019
JournalPedosphere
Journal citation29 (3), pp. 360-373
Digital Object Identifier (DOI)doi:10.1016/S1002-0160(17)60370-9
Open accessPublished as non-open access
Publisher's version1-s2.0-S1002016017603709-main (1).pdf
Output statusPublished
Publication dates
Online18 Apr 2018
Publication process dates
Accepted18 Apr 2018
PublisherScience Press
ISSN1002-0160

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