Systematic Y2H Screening Reveals Extensive Effector-Complex Formation

A - Papers appearing in refereed journals

Alcantara, A., Bosch, J., Nazari, F., Hoffmann, G., Gallei, M., Uhse, S., Darino, M.A., Olukayode, T., Reumann, D., Baggaley, L. and Djamei, A. 2019. Systematic Y2H Screening Reveals Extensive Effector-Complex Formation. Frontiers in Plant Science. 10 (1437).

AuthorsAlcantara, A., Bosch, J., Nazari, F., Hoffmann, G., Gallei, M., Uhse, S., Darino, M.A., Olukayode, T., Reumann, D., Baggaley, L. and Djamei, A.
Abstract

During infection pathogens secrete small molecules, termed effectors, to manipulate and control the interaction with their specific hosts. Both the pathogen and the plant are under high selective pressure to rapidly adapt and co-evolve in what is usually referred to as molecular arms race. Components of the host’s immune system form a network that processes information about molecules with a foreign origin and damage-associated signals, integrating them with developmental and abiotic cues to adapt the plant’s responses. Both in the case of nucleotide-binding leucine-rich repeat receptors and leucine-rich repeat receptor kinases interaction networks have been extensively characterized. However, little is known on whether pathogenic effectors form complexes to overcome plant immunity and promote disease. Ustilago maydis, a biotrophic fungal pathogen that infects maize plants, produces effectors that target hubs in the immune network of the host cell. Here we assess the capability of U. maydis effector candidates to interact with each other, which may play a crucial role during the infection process. Using a systematic yeast-two-hybrid approach and based on a preliminary pooled screen, we selected 63 putative effectors for one-on-one matings with a library of nearly 300 effector candidates. We found that 126 of these effector candidates interacted either with themselves or other predicted effectors. Although the functional relevance of the observed interactions remains elusive, we propose that the observed abundance in complex formation between effectors adds an additional level of complexity to effector research and should be taken into consideration when studying effector evolution and function. Based on this fundamental finding, we suggest various scenarios which could evolutionarily drive the formation and stabilization of an effector interactome.

KeywordsProtein-protein interaction network; Effector proteins; Ustilago maydis; Plant pathogen; Yeast-two-hybrid
Year of Publication2019
JournalFrontiers in Plant Science
Journal citation10 (1437)
Digital Object Identifier (DOI)doi:10.3389/fpls.2019.01437
PubMed ID 31803201
PubMed Central IDPMC6872519
Web address (URL)https://www.frontiersin.org/articles/10.3389/fpls.2019.01437/full
Open accessPublished as ‘gold’ (paid) open access
Publisher's version
Copyright license
Author copyright
Supplemental file
Copyright license
Author copyright
Output statusPublished
Publication dates
Online14 Nov 2019
Publication process dates
Accepted16 Oct 2019
PublisherFrontiers Media SA
ISSN1664-462X

Permalink - https://repository.rothamsted.ac.uk/item/97109/systematic-y2h-screening-reveals-extensive-effector-complex-formation

2 total views
0 total downloads
1 views this month
0 downloads this month
Download files as zip