Phylogeny and gene expression of the complete NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY (NPF) in Triticum aestivum L.

A - Papers appearing in refereed journals

Wang, H., Wan, Y., Buchner, P. H., King, R., Ma, H. and Hawkesford, M. J. 2020. Phylogeny and gene expression of the complete NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY (NPF) in Triticum aestivum L. Journal of Experimental Botany. p. eraa210. https://doi.org/10.1093/jxb/eraa210

AuthorsWang, H., Wan, Y., Buchner, P. H., King, R., Ma, H. and Hawkesford, M. J.
Abstract

NPF genes encode membrane transporters involved in the transport of a large variety of substrates including nitrate and peptides. The NPF gene family has been described for many plants, but the whole NPF gene family for wheat has not been completely identified. The release of the wheat reference genome has enabled the identification of the entire wheat NPF gene family. A systematic analysis of the whole wheat NPF gene family was performed, including responses of specific gene expression to development and nitrogen supply. A total of 331 NPF genes (113 homoeologous groups) have been identified in wheat. The chromosomal location of the NPF genes is unevenly distributed with predominant occurrence in the long arms of the chromosomes. The phylogenetic analysis indicated that wheat NPF genes are closely clustered with Arabidopsis, Brachypodium and rice orthologs, and subdivided into eight subfamilies. The expression profiles of wheat NPF genes were examined using RNA-seq data and identified a subset of 44 NPF genes (homoeologous groups) with contrasting expression responses to nitrogen and/or development in different tissues. The systematic identification of gene composition, chromosomal locations, evolutionary relationships and expression profiles contributes to a better understanding of the roles of the wheat NPF genes and lays the foundation for further functional analysis in wheat.

KeywordsNPF gene; Nitrate; Nitrogen; Wheat; Gene expression; Phlogeny
Year of Publication2020
JournalJournal of Experimental Botany
Journal citationp. eraa210
Digital Object Identifier (DOI)https://doi.org/10.1093/jxb/eraa210
Open accessPublished as bronze (free) open access
FunderBiotechnology and Biological Sciences Research Council
Department of Environment, Food and Rural Affairs
Funder project or codeDesigning Future Wheat - WP1 - Increased efficiency and sustainability
The Wheat Genetic Improvement Network (WGIN) - Improving the environmental footprint of farming through crop genetics and targeted traits analysis
Publisher's version
Output statusPublished
Publication dates
Online28 May 2020
Publication process dates
Accepted27 Apr 2020
PublisherOxford University Press (OUP)
ISSN0022-0957

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