Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1

A - Papers appearing in refereed journals

Snell, P., Wilkinson, M. D., Taylor, G. J., Hall, S., Sharma, S., Sirijovski, N., Hansson, M., Shewry, P. R., Hofvander, P. and Grimberg, A. 2022. Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1. Plants - Basel. 11 (7), p. 889. https://doi.org/10.3390/plants11070889

AuthorsSnell, P., Wilkinson, M. D., Taylor, G. J., Hall, S., Sharma, S., Sirijovski, N., Hansson, M., Shewry, P. R., Hofvander, P. and Grimberg, A.
Abstract

Wheat (Triticum aestivum L.) is one of the major staple crops in the world and is used to prepare a range of foods. The development of new varieties with wider variation in grain composition could broaden their use. We characterized grains and flours from oil-accumulating transgenic wheat expressing the oat (Avena sativa L.) endosperm WRINKLED1 (AsWRI1) grown under field conditions. Lipid and starch accumulation was determined in developing caryopses of AsWRI1-wheat and X-ray microtomography was used to study grain morphology. The developing caryopses of AsWRI1-wheat grains had increased triacylglycerol content and decreased starch content compared to the control. Mature AsWRI1-wheat grains also had reduced weight, were wrinkled and had a shrunken endosperm and X-ray tomography revealed that the proportion of endosperm was decreased while that of the aleurone was increased. Grains were milled to produce two white flours and one bran fraction. Mineral and lipid analyses showed that the flour fractions from the AsWRI1-wheat were contaminated with bran, due to the effects of the changed morphology on milling. This study gives a detailed analysis of grains from field grown transgenic wheat that expresses a gene that plays a central regulatory role in carbon allocation and significantly affects grain composition.

KeywordsAleurone cells; Triticum aestivum; Carbon allocation; Cereal endosperm; Oil biosynthesis; Transcription factor; Wheat flour; WRINKLED1; Triacylglycerol
Year of Publication2022
JournalPlants - Basel
Journal citation11 (7), p. 889
Digital Object Identifier (DOI)https://doi.org/10.3390/plants11070889
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
Publisher's version
Output statusPublished
Publication dates
Online26 Mar 2022
Publication process dates
Accepted23 Mar 2022
PublisherMDPI
ISSN2223-7747

Permalink - https://repository.rothamsted.ac.uk/item/98854/characterisation-of-grains-and-flour-fractions-from-field-grown-transgenic-oil-accumulating-wheat-expressing-oat-wri1

17 total views
1 total downloads
4 views this month
0 downloads this month
Download files as zip