Chickpea Defensin Gene Family: Promising Candidates for Resistance Against Soil‑Borne Chickpea Fungal Pathogens

A - Papers appearing in refereed journals

Nitnavare, R., Pothana, A., Yeshvekar, R. K., Bhattacharya, J., Sapara, V., Reddy, P. S., Ramtirtha, Y., Tarafdar, A., Sharma, M. and Bhatnagar‑Mathur, P. 2022. Chickpea Defensin Gene Family: Promising Candidates for Resistance Against Soil‑Borne Chickpea Fungal Pathogens. Journal of Plant Growth Regulation. https://doi.org/10.1007/s00344-022-10811-1

AuthorsNitnavare, R., Pothana, A., Yeshvekar, R. K., Bhattacharya, J., Sapara, V., Reddy, P. S., Ramtirtha, Y., Tarafdar, A., Sharma, M. and Bhatnagar‑Mathur, P.
Abstract

Defensins are broad-spectrum antimicrobial peptides that play an important role in providing innate immunity to various
biotic stresses in plants. We identifed and characterized 22 defensin (DEF) and defensin-like (DEFL) genes in chickpea
(Cicer arientinum) based on their structures, expression, chromosomal localization, conserved motifs, and cis-regulatory elements. The localization of DEF and DEFL genes in chickpea genome revealed the presence of at least two clusters that are likely evolved through local gene duplications. Genotype-specifc responses of several CaDEF and CaDEFL genes in fungal bioassays suggested their involvement in defense against fungal pathogens such as hemi-biotrophic F. oxysporum f.
sp. ciceris and dry root rot causing necrotrophic R. bataticola. Molecular docking studies revealed interactions of CaDEFs with fungal plasma membrane components such as phosphatidylserine (PS) and glucosylceramide (GluCer) and their binding sites were identifed. Our data will be useful to identify potential candidate genes and their role in host-plant resistance in chickpea, besides presenting opportunities for their potential for possible deployment in other crops

KeywordsAnti-fungal proteins (AFPs) ; Chickpea; Defensins; Dry root rot; Fusarium; Host pathogen interactions
Year of Publication2022
JournalJournal of Plant Growth Regulation
Digital Object Identifier (DOI)https://doi.org/10.1007/s00344-022-10811-1
Web address (URL)https://link.springer.com/article/10.1007/s00344-022-10811-1
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeS2N - Soil to Nutrition [ISPG]
Output statusPublished
Publication dates
Online03 Oct 2022
Publication process dates
Accepted15 Aug 2022
PublisherSpringer
ISSN0721-7595

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