Nitrogen uptake and remobilization from pre‑ and post‑anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditions

A - Papers appearing in refereed journals

Sharma, S., Kumar, T., Foulkes, M. J., Orford, S, Singh, A. M., Wingen, L. U., Venkatesh, K., Lekshmy, S. N., Mandal, P. K., Griffiths, S., Hawkesford, M. J., Shewry, P. R., Bentley, A. R. and Pandey, R. 2023. Nitrogen uptake and remobilization from pre‑ and post‑anthesis stages contribute towards grain yield and grain protein concentration in wheat grown in limited nitrogen conditions. CABI Agriculture and Bioscience. 4 (1), p. 12. https://doi.org/10.1186/s43170-023-00153-7

AuthorsSharma, S., Kumar, T., Foulkes, M. J., Orford, S, Singh, A. M., Wingen, L. U., Venkatesh, K., Lekshmy, S. N., Mandal, P. K., Griffiths, S., Hawkesford, M. J., Shewry, P. R., Bentley, A. R. and Pandey, R.
Abstract

Background
In wheat, nitrogen (N) remobilization from vegetative tissues to developing grains largely depends on genetic and environmental factors. The evaluation of genetic potential of crops under limited resource inputs such as limited N supply would provide an opportunity to identify N-efficient lines with improved N utilisation efficiency and yield potential. We assessed the genetic variation in wheat recombinant inbred lines (RILs) for uptake, partitioning, and remobilization of N towards grain, its association with grain protein concentration (GPC) and grain yield.

Methods
We used the nested association mapping (NAM) population (195 lines) derived by crossing Paragon (P) with CIMMYT core germplasm (P × Cim), Baj (P × Baj), Watkins (P × Wat), and Wyalkatchem (P × Wya). These lines were evaluated in the field for two seasons under limited N supply. The plant sampling was done at anthesis and physiological maturity stages. Various physiological traits were recorded and total N uptake and other N related indices were calculated. The grain protein deviation (GPD) was calculated from the regression of grain yield on GPC. These lines were grouped into different clusters by hierarchical cluster analysis based on grain yield and N-remobilization efficiency (NRE).

Results
The genetic variation in accumulation of biomass at both pre- and post-anthesis stages were correlated with grain-yield. The NRE significantly correlated with aboveground N uptake at anthesis (AGNa) and grain yield but negatively associated with AGN at post-anthesis (AGNpa) suggesting higher N uptake till anthesis favours high N remobilization during grain filling. Hierarchical cluster analysis of these RILs based on NRE and yield resulted in four clusters, efficient (31), moderately efficient (59), moderately inefficient (58), and inefficient (47). In the N-efficient lines, AGNa contributed to 77% of total N accumulated in grains, while it was 63% in N-inefficient lines. Several N-efficient lines also exhibited positive grain protein deviation (GPD), combining high grain yield and GPC. Among crosses, the P × Cim were superior and N-efficient, while P × Wya responded poorly to low N input.

KeywordsBiomass partitioning; Grain protein concentration; Grain protein deviation; Low soil nitrogen; Nitrogen remobilization efficiency; Triticum aestivum
Year of Publication2023
JournalCABI Agriculture and Bioscience
Journal citation4 (1), p. 12
Digital Object Identifier (DOI)https://doi.org/10.1186/s43170-023-00153-7
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeIndo-UK Centre for the improvement of Nitrogen use Efficiency in Wheat (INEW)
Designing Future Wheat - WP1 - Increased efficiency and sustainability
Designing Future Wheat WP2.2
Publisher's version
Output statusPublished
Publication dates
Online04 May 2023
Publication process dates
Accepted24 Apr 2023
PublisherCAB International (CABI)
ISSN2662-4044

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