The genotype of barley cultivars influences multiple aspects of their associated microbiota via differential root exudate secretion

A - Papers appearing in refereed journals

Pacheco-Moreno, A., Bollmann-Giolai, A., Chandra, G., Brett, P., Davies, J., Thornton, O., Poole, P., Ramachandran, V., Brown, J. K. M., Nicholson, P., Ridout, C., DeVos, S. and Malone, J. G. 2024. The genotype of barley cultivars influences multiple aspects of their associated microbiota via differential root exudate secretion. PLOS Biology. 22 (4), p. e3002232. https://doi.org/10.1371/journal.pbio.3002232

AuthorsPacheco-Moreno, A., Bollmann-Giolai, A., Chandra, G., Brett, P., Davies, J., Thornton, O., Poole, P., Ramachandran, V., Brown, J. K. M., Nicholson, P., Ridout, C., DeVos, S. and Malone, J. G.
Abstract

Plant-associated microbe play vital roles in promoting plant growth and health, with plants secreting root exudates into the rhizosphere to attract beneficial microbes. Exudate composition defines the nature of microbial recruitment, with different plant species attracting distinct microbiota to enable optimal adaptation to the soil environment. To more closely examine the relationship between plant genotype and microbial recruitment, we analysed the rhizosphere microbiomes of landrace (Chevallier) and modern (NFC Tipple) barley (Hordeum vulgare) cultivars. Distinct differences were observed between the plant associated microbiomes of the 2 cultivars, with the plant-growth promoting rhizobacterial genus Pseudomonas substantially more abundant in the Tipple rhizosphere. Striking differences were also observed between the phenotypes of recruited Pseudomonas populations, alongside distinct genotypic clustering by cultivar. Cultivar-driven Pseudomonas selection was driven by root exudate composition, with the greater abundance of hexose sugars secreted from Tipple roots attracting microbes better adapted to growth on these metabolites and vice versa. Cultivar-driven selection also operates at the molecular level, with both gene expression and the abundance of ecologically relevant loci differing between Tipple and Chevallier Pseudomonas isolates. Finally, cultivar-driven selection is important for plant health, with both cultivars showing a distinct preference for microbes selected by their genetic siblings in rhizosphere transplantation assays

Year of Publication2024
JournalPLOS Biology
Journal citation22 (4), p. e3002232
Digital Object Identifier (DOI)https://doi.org/10.1371/journal.pbio.3002232
Open accessPublished as ‘gold’ (paid) open access
FunderGlobal Challenges Research Fund (UKRI)
Funder project or codeBB/M011216/1
BBS/E/J/000PR9797
BB/T001801/1
BB/K02003X/1
Publisher's version
Output statusPublished
Publication dates
Online25 Apr 2024
Publication process dates
Accepted19 Mar 2024
ISSN1544-9173
PublisherPublic Library of Science (PLOS)

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