Genome-wide screening and characterization of phospholipase A (PLA)-like genes in sorghum (Sorghum bicolor L.).

A - Papers appearing in refereed journals

Sapara, V. J., Shankhapal, A. and Reddy, A. R. 2024. Genome-wide screening and characterization of phospholipase A (PLA)-like genes in sorghum (Sorghum bicolor L.). Planta. 260, p. 35. https://doi.org/10.1007/s00425-024-04467-2

AuthorsSapara, V. J., Shankhapal, A. and Reddy, A. R.
Abstract

Sorghum bicolor (L.) Moench is the fifth most cultivated crop worldwide, and it is used in many ways, but it has always gained less popularity due to the yield, pest, and environmental constraints. Improving genetic background and developing better varieties is crucial for better sorghum production in semi-arid tropical regions. This study focuses on the phospholipase A (PLA) family within sorghum, comprehensively characterising PLA genes and their expression across different tissues. The investigation identified 32 PLA genes in the sorghum genome, offering insights into their chromosomal localization, molecular weight, isoelectric point, and subcellular distribution through bioinformatics tools. PLA-like family genes are classified into three groups, namely patatin-related phospholipase A (pPLA), phospholipase A1 (PLA1), and phospholipase A2 (PLA2). In-silico chromosome localization studies revealed that these genes are unevenly distributed in the sorghum genome. Cis-motif analysis revealed the presence of several developmental, tissue and hormone-specific elements in the promoter regions of the PLA genes. Expression studies in different tissues such as leaf, root, seedling, mature seed, immature seed, anther, and pollen showed differential expression patterns. Taken together, genome-wide analysis studies of PLA genes provide a better understanding and critical role of this gene family considering the metabolic processes involved in plant growth, defence and stress response.

KeywordsBioinformatics tools ; Cis-motif; Chromosomal localization; Gene expression studies; In-silico; Metabolic process; Sorghum genome
Year of Publication2024
JournalPlanta
Journal citation260, p. 35
Digital Object Identifier (DOI)https://doi.org/10.1007/s00425-024-04467-2
Open accessPublished as non-open access
Funder project or codeUnderstanding mesocotyl elongation in rice
Output statusPublished
Publication dates
Print26 Jun 2024
Publication process dates
Accepted13 Jun 2024
PublisherSpringer
ISSN0032-0935

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