A - Papers appearing in refereed journals
Tang, S., Pan, W., Zhou, J., Ma, Q., Yang, X., Wanek, W., Marsden, K. A., Kuzyakov, Y., Chadwick, D. R., Wu, L., Gregory, A. S. and Jones, D. L. 2024. Soil nitrogen and phosphorus regulate decomposition of organic nitrogen compounds in the rothamsted experiment. Soil Biology and Biochemistry. 196, p. 109502. https://doi.org/10.1016/j.soilbio.2024.109502
Authors | Tang, S., Pan, W., Zhou, J., Ma, Q., Yang, X., Wanek, W., Marsden, K. A., Kuzyakov, Y., Chadwick, D. R., Wu, L., Gregory, A. S. and Jones, D. L. |
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Abstract | The effects of long-term N and P fertilisation on soil organic nitrogen (SON) turnover are unclear. We sampled soils fertilised by N and/or P since 1843 at Rothamsted Research to investigate the effects of long-term N and P fertilisation on high- or low-molecular-weight SON decomposition and gross N mineralisation. Short-term assays with added 14C-labelled proteins, peptides, and amino acids and gross organic N mineralisation were measured, and the microbial communities and functions were also assessed in parallel. Long-term N fertilisation increased the contents of soil extractable organic N and peptides but decreased organic N-hydrolysing enzyme activities (N-acetyl-β-D-glucosaminidase and leucine aminopeptidase). Fertilisation with P and N accelerated and reduced the decomposition of 14C-labelled organic N compounds, respectively. The decomposition of peptides and amino acids, as labile SON components, was mainly regulated by soil P content because microbial biomass and activity were more sensitive to P fertilisation than to N fertilisation. Gross NH4+/NO3− production and consumption were accelerated by 41%–60% under N fertilisation but remained unchanged under P fertilisation compared to the unfertilised treatment. Metagenomic sequencing showed that N fertilisation increased microbial diversity and enriched functional genes associated with organic N decomposition, compared to the unfertilised treatment. P fertilisation had no effect on the abundance of these functional genes. In agricultural practices, it is essential to comprehensively consider the interaction between N and P fertilisation to optimise the cycling and utilisation of SON. |
Keywords | Long-term experiment; Nitrogen fertilisation; Phosphorus fertilisation; Soil organic nitrogen decomposition; Isotope labelling |
Year of Publication | 2024 |
Journal | Soil Biology and Biochemistry |
Journal citation | 196, p. 109502 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.soilbio.2024.109502 |
Web address (URL) | https://doi.org/10.1016/j.soilbio.2024.109502 |
Open access | Published as non-open access |
Funder | National Key Technologies R&D Program of China |
National Natural Science Foundation of China | |
Biotechnology and Biological Sciences Research Council | |
Funder project or code | BBSRC National Bioscience Research Infrastructure: Rothamsted Long-Term Experiments |
Output status | Published |
Publication dates | |
Online | 18 Jun 2024 |
Publication process dates | |
Accepted | 17 Jun 2024 |
Publisher | Elsevier |
ISSN | 0038-0717 |
Permalink - https://repository.rothamsted.ac.uk/item/992x8/soil-nitrogen-and-phosphorus-regulate-decomposition-of-organic-nitrogen-compounds-in-the-rothamsted-experiment
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