In vitro fermentability of xylo-oligosaccharide and xylo-polysaccharide fractions with different molecular weights by human faecal bacteria

A - Papers appearing in refereed journals

Ho, A. L., Kosik, O., Lovegrove, A., Charalampopoulos, D and Rastall, R. A. 2018. In vitro fermentability of xylo-oligosaccharide and xylo-polysaccharide fractions with different molecular weights by human faecal bacteria. Carbohydrate Polymers. 190, pp. 50-58.

AuthorsHo, A. L., Kosik, O., Lovegrove, A., Charalampopoulos, D and Rastall, R. A.
Abstract

Xylo-oligosaccharides and xylo-polysaccharides (XOS, XPS) produced by autohydrolysis of the fibre from oil palm empty fruit bunches (OPEFB) were purified using gel filtration chromatography to separate the XOS and XPS from the crude autohydrolysis liquor. Six mixed fractions of refined XOS and XPS with average degree of polymerisation (avDP) of 4–64 were obtained. These were characterised in terms of their composition and size by HPLC, MALDI-ToF-MS (selected fractions) and carbohydrate gel electrophoresis (PACE). They were assessed in batch culture fermentations using faecal inocula to determine their ability to modulate the human faecal microbiota in vitro by measuring the bacterial growth, organic acid production and the XOS assimilation profile. The gut microbiota was able to utilise all the substrates and there was a link between the avDP with the fermentation properties. In general, XOS/XPS preparations of lower avDP promote better Bifidobacterium growth and organic acid production.

KeywordsAutohydrolysis; In vitro fermentation; Oil palm empty fruit bunches; Xylo-oligo and xylo-polysaccharides
Year of Publication2018
JournalCarbohydrate Polymers
Journal citation190, pp. 50-58
Digital Object Identifier (DOI)doi:10.1016/j.carbpol.2017.08.077
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Malaysian Ministry of Higher Education
Funder project or codeDevelopment of a process scheme for the production of high value functional products from DDGS
Output statusPublished
Publication dates
Print01 Jan 2018
Online06 Sep 2017
Publication process dates
Accepted17 Aug 2017
PublisherElsevier Sci Ltd
Copyright licenseCC BY
ISSN0144-8617

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