Molecular cloning of beta-kafirin, a methionine-rich protein of sorghum grain

A - Papers appearing in refereed journals

Chamba, E. B., Halford, N. G., Forsyth, J., Wilkinson, M. D. and Shewry, P. R. 2005. Molecular cloning of beta-kafirin, a methionine-rich protein of sorghum grain. Journal of Cereal Science. 41 (3), pp. 381-383.

AuthorsChamba, E. B., Halford, N. G., Forsyth, J., Wilkinson, M. D. and Shewry, P. R.
Abstract

Eight independantly amplified clones, four each from genomic DNA and cDNA, were sequenced. They encoded beta-kafirin proteins with identical amino acid sequences, the mature proteins comprising 172 amino acids including 16 methionine residues and 8 cysteine residues. The identical sequences of the clones and the results of Southern blot analysis were consistent with the presence of a single gene copy. Northern blotting showed that the transcripts were present in developing seeds between 14 and 35 days after anthesis.

KeywordsSorghum; Kafirin; Methionine; Nutritional quality
Year of Publication2005
JournalJournal of Cereal Science
Journal citation41 (3), pp. 381-383
Digital Object Identifier (DOI)doi:10.1016/j.jcs.2004.09.004
Open accessPublished as non-open access
Funder project or code501
Output statusPublished
Publication dates
Print01 May 2005
Publication process dates
Accepted30 Sep 2004
Copyright licensePublisher copyright
ISSN0733-5210
PublisherAcademic Press Ltd- Elsevier Science Ltd

Permalink - https://repository.rothamsted.ac.uk/item/8969q/molecular-cloning-of-beta-kafirin-a-methionine-rich-protein-of-sorghum-grain

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