Molecular cloning of beta-kafirin, a methionine-rich protein of sorghum grain

A - Papers appearing in refereed journals

Chamba, E. B., Halford, N. G., Forsyth, J., Wilkinson, M. D. and Shewry, P. R. 2005. Molecular cloning of beta-kafirin, a methionine-rich protein of sorghum grain. Journal of Cereal Science. 41 (3), pp. 381-383. https://doi.org/10.1016/j.jcs.2004.09.004

AuthorsChamba, E. B., Halford, N. G., Forsyth, J., Wilkinson, M. D. and Shewry, P. R.
Abstract

Eight independantly amplified clones, four each from genomic DNA and cDNA, were sequenced. They encoded beta-kafirin proteins with identical amino acid sequences, the mature proteins comprising 172 amino acids including 16 methionine residues and 8 cysteine residues. The identical sequences of the clones and the results of Southern blot analysis were consistent with the presence of a single gene copy. Northern blotting showed that the transcripts were present in developing seeds between 14 and 35 days after anthesis.

KeywordsSorghum; Kafirin; Methionine; Nutritional quality
Year of Publication2005
JournalJournal of Cereal Science
Journal citation41 (3), pp. 381-383
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jcs.2004.09.004
Open accessPublished as non-open access
Funder project or code501
Structural and protein engineering studies of novel cereal and oilseed proteins with functional properties or biological activity
Output statusPublished
Publication dates
Print01 May 2005
Publication process dates
Accepted30 Sep 2004
Copyright licensePublisher copyright
ISSN0733-5210
PublisherAcademic Press Ltd- Elsevier Science Ltd

Permalink - https://repository.rothamsted.ac.uk/item/8969q/molecular-cloning-of-beta-kafirin-a-methionine-rich-protein-of-sorghum-grain

Restricted files

Publisher's version

Under embargo indefinitely

548 total views
1 total downloads
2 views this month
0 downloads this month