Purification and characterisation of a panel of peanut allergens suitable for use in allergy diagnosis

A - Papers appearing in refereed journals

Marsh, J., Rigby, N., Wellner, K., Reese, G., Knulst, A., Akkerdaas, J., Van Ree, R., Radauer, C., Lovegrove, A., Sancho, A., Mills, C., Vieths, S., Hoffmann-Sommergruber, K. and Shewry, P. R. 2008. Purification and characterisation of a panel of peanut allergens suitable for use in allergy diagnosis. Molecular Nutrition & Food Research. 52(Supplement 2), pp. S272-S285.

AuthorsMarsh, J., Rigby, N., Wellner, K., Reese, G., Knulst, A., Akkerdaas, J., Van Ree, R., Radauer, C., Lovegrove, A., Sancho, A., Mills, C., Vieths, S., Hoffmann-Sommergruber, K. and Shewry, P. R.
Abstract

Peanut is a major cause of type 1 hypersensitive reactions including anaphylaxis. This results from the presence of a number of protein allergens, six of which are being studied as part of the EU FP6 EuroPrevall programme. These are Ara h 1 (7S globulin), Ara h 2, Ara h 6 (2S albumins), Ara h 3/4 (11S globulins) and Ara h 8 (Bet v 1 homologue). Methods for the purification of Ara h 1, Ara h 3/4, Ara h 2 and Ara h 6 from peanut seeds and for the production of recombinant Ara h 8 in Escherichia coli are described with spectroscopic analyses being used to confirm that they are authentically folded. N-terminal sequencing of the proteins purified from peanut seeds also revealed details of the differences between isoforms and their generation by proteolytic processing within the seed. Preliminary IgE binding studies of the purified allergens confirmed that they retained their immunological properties indicating their suitability for use in allergy diagnosis.

KeywordsFood Science & Technology
Year of Publication2008
JournalMolecular Nutrition & Food Research
Journal citation52(Supplement 2), pp. S272-S285
Digital Object Identifier (DOI)doi:10.1002/mnfr.200700524
PubMed ID18727014
Open accessPublished as non-open access
FunderEuropean Union - EU
Funder project or codeCentre for Crop Genetic Improvement (CGI)
ISSN16134125
PublisherWiley
Grant ID51400

Permalink - https://repository.rothamsted.ac.uk/item/8q099/purification-and-characterisation-of-a-panel-of-peanut-allergens-suitable-for-use-in-allergy-diagnosis

23 total views
0 total downloads
7 views this month
0 downloads this month