Genetic dissection of basal defence responsiveness in accessions of Arabidopsis thaliana

A - Papers appearing in refereed journals

Ahmad, S., Van Hulten, M., Martin, J. L., Pieterse, C. M. J., Van Wees, S. C. M. and Ton, J. 2011. Genetic dissection of basal defence responsiveness in accessions of Arabidopsis thaliana. Plant, Cell & Environment. 34 (7), pp. 1191-1206.

AuthorsAhmad, S., Van Hulten, M., Martin, J. L., Pieterse, C. M. J., Van Wees, S. C. M. and Ton, J.
Abstract

Basal resistance involves a multitude of pathogen- and herbivore-inducible defence mechanisms, ranging from localized callose deposition to systemic defence gene induction by salicylic acid (SA) and jasmonic acid (JA). In this study, we have explored and dissected genetic variation in the responsiveness of basal defence mechanisms within a selection of Arabidopsis accessions. Responsiveness of JA-induced PDF1.2 gene expression was associated with enhanced basal resistance against the necrotrophic fungus Plectosphaerella cucumerina and the herbivore Spodoptera littoralis. Conversely, accessions showing augmented PR-1 induction upon SA treatment were more resistant to the hemi-biotrophic pathogen Pseudomonas syringae, and constitutively expressed defence-related transcription factor (TF) genes. Unexpectedly, accessions with primed responsiveness to SA deposited comparatively little callose after treatment with microbe-associated molecular patterns. A quantitative trait locus (QTL) analysis identified two loci regulating flagellin-induced callose and one locus regulating SA-induced PR-1 expression. The latter QTL was found to contribute to basal resistance against P. syringae. None of the defence regulatory QTLs influenced plant growth, suggesting that the constitutive defence priming conferred by these loci is not associated with major costs on plant growth. Our study demonstrates that natural variation in basal resistance can be exploited to identify genetic loci that prime the plant's basal defence arsenal.

KeywordsPlant Sciences
Year of Publication2011
JournalPlant, Cell & Environment
Journal citation34 (7), pp. 1191-1206
Digital Object Identifier (DOI)doi:10.1111/j.1365-3040.2011.02317.x
PubMed ID21414016
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Netherlands Organization for Scientific Research ( NWO)
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
Publisher's version
PublisherWiley
Wiley-Blackwell
Grant IDBB/E023959/1
865.04.002, 863.04.019
ISSN0140-7791

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