A - Papers appearing in refereed journals
Anders, N., Wilkinson, M. D., Lovegrove, A., Freeman, J., Tryfona, T., Pellny, T. K., Weimar, T., Mortimer, J. C., Stott, K., Baker, J. M., Defoin-Platel, M., Shewry, P. R., Dupree, P. and Mitchell, R. A. C. 2012. Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses. Proceedings of the National Academy of Sciences of the United States of America. 109 (3), pp. 989-993. https://doi.org/10.1073/pnas.1115858109
Authors | Anders, N., Wilkinson, M. D., Lovegrove, A., Freeman, J., Tryfona, T., Pellny, T. K., Weimar, T., Mortimer, J. C., Stott, K., Baker, J. M., Defoin-Platel, M., Shewry, P. R., Dupree, P. and Mitchell, R. A. C. |
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Abstract | Xylan, a hemicellulosic component of the plant cell wall, is one of the most abundant polysaccharides in nature. In contrast to dicots, xylan in grasses is extensively modified by α-(1,2)– and α-(1,3)–linked arabinofuranose. Despite the importance of grass arabinoxylan in human and animal nutrition and for bioenergy, the enzymes adding the arabinosyl substitutions are unknown. Here we demonstrate that knocking-down glycosyltransferase (GT) 61 expression in wheat endosperm strongly decreases α-(1,3)–linked arabinosyl substitution of xylan. Moreover, heterologous expression of wheat and rice GT61s in Arabidopsis leads to arabinosylation of the xylan, and therefore provides gain-of-function evidence for α-(1,3)-arabinosyltransferase activity. Thus, GT61 proteins play a key role in arabinoxylan biosynthesis and therefore in the evolutionary divergence of grass cell walls. |
Keywords | RRES175; 175_Biochemistry; 175_Climatology; 175_Plant sciences; 175_Genetics |
Year of Publication | 2012 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Journal citation | 109 (3), pp. 989-993 |
Digital Object Identifier (DOI) | https://doi.org/10.1073/pnas.1115858109 |
Open access | Published as bronze (free) open access |
Funder project or code | Centre for Crop Genetic Improvement (CGI) |
Centre for Mathematical and Computational Biology (MCB) | |
Manipulation of cell wall synthesis to improve the dietary fibre composition of wheat flour | |
Project: 4675 | |
Publisher's version | |
Publication process dates | |
Accepted | 07 Dec 2011 |
Publisher | National Academy of Sciences |
National Academy of Sciences of the United States of America | |
Copyright license | Publisher copyright |
ISSN | 0027-8424 |
Permalink - https://repository.rothamsted.ac.uk/item/8q9w9/glycosyl-transferases-in-family-61-mediate-arabinofuranosyl-transfer-onto-xylan-in-grasses