The effects of sulphur starvation on the amino acid and protein compositions of barley grain

A - Papers appearing in refereed journals

Shewry, P. R., Franklin, J., Parmar, S., Smith, S. J. and Miflin, B. J. 1983. The effects of sulphur starvation on the amino acid and protein compositions of barley grain. Journal of Cereal Science. 1 (1), pp. 21-31.

AuthorsShewry, P. R., Franklin, J., Parmar, S., Smith, S. J. and Miflin, B. J.
Abstract

Sulphur starvation resulted in decreases in the total sulphur content, and in the proportions of the sulphur-containing amino acids, cysteine and methionine, in the grain of pot-grown plants of the barley cultivars Sundance and Athos. The S-deficient grain contained a decreased proportion of storage protein (hordein) and increased non-protein nitrogen (NPN). Comparison of the amino acid compositions of the whole grain, and of the individual protein fractions, indicated that aspartate or asparagine was a major component of the increased NPN. Electrophoretic analyses showed that the hordein fraction was depleted in S-rich ‘B’ and ‘D’ hordein polypeptides, which was consistent with its low content of sulphur-containing amino acids. The salt-soluble protein fraction also contained low levels of cysteine and methionine, and SDS-PAGE showed that some bands, notably those of low molecular weight, were either greatly reduced in amount or were absent. It is suggested that these are non-essential components, possibly storage proteins. In contrast there was little effect on the amino acid or polypeptide compositions of the glutelin fraction.

Year of Publication1983
JournalJournal of Cereal Science
Journal citation1 (1), pp. 21-31
Digital Object Identifier (DOI)doi:10.1016/s0733-5210(83)80005-8
Open accessPublished as non-open access
Output statusPublished
Publication dates
Online07 Sep 2009
Publication process dates
Accepted19 Aug 1982
Copyright licensePublisher copyright
PublisherElsevier
Academic Press Ltd- Elsevier Science Ltd
ISSN0733-5210

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