Serological studies on sugar-beet yellows virus

A - Papers appearing in refereed journals

Kleczkowski, A. and Watson, M. A. 1944. Serological studies on sugar-beet yellows virus. Annals of Applied Biology - AAB. 31 (2), pp. 116-120. https://doi.org/10.1111/j.1744-7348.1944.tb06221.x

AuthorsKleczkowski, A. and Watson, M. A.
Abstract

Specific antisera have been prepared against the sap expressed from beet plants infected with beet yellows virus.

The antigen is unstable. In the sap it is destroyed by keeping for 2‐3 days at room temperature or by heating for 10 min. at 52d̀C. It is unaffected by pH changes between 5 and 9. In detached leaves at room temperature it remains unchanged for at least 6 days, whereas the ability of aphides to transmit from these leaves fell considerably in 4 days.

The antigen can be reversibly precipitated by ammonium sulphate or sedimented by high‐speed centrifugation. However, all the attempts to isolate it from other sap constituents or concentrate it have failed.

The precipitin reaction is of value for diagnosis, and works successfully with crude sap.

Year of Publication1944
JournalAnnals of Applied Biology - AAB
Journal citation31 (2), pp. 116-120
Digital Object Identifier (DOI)https://doi.org/10.1111/j.1744-7348.1944.tb06221.x
Open accessPublished as non-open access
Output statusPublished
Publication dates
Print01 May 1944
Online26 Feb 2008
Copyright licensePublisher copyright
ISSN0003-4746
PublisherWiley

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