Fixation and electron microscopy of the Rothamsted culture of henbane mosaic virus

A - Papers appearing in refereed journals

Plumb, R. T. and Vince, D. A. 1971. Fixation and electron microscopy of the Rothamsted culture of henbane mosaic virus. Journal of General Virology. 13 (Nov), pp. 357-359.

AuthorsPlumb, R. T. and Vince, D. A.

Thin sections of tissue infected by viruses of the potato virus Y (PVY) group often fail to show virus particles. Henbane mosaic virus (HMV) is more concentrated in infected tissue than most PVY group viruses and seemed a suitable subject for electron microscopy. We failed to detect any particles after tissues were subjected to a commonly used double fixation technique but found them in tissue fixed with osmic acid; parallel results were found when purified virus preparations were similarly treated.

This paper reports some details of the fine structure of infected tissue and the effect of fixation on virus within the host cell and in purified preparations.

The Rothamsted culture of HMV used has been maintained in Nicotiana tabacum, cv. White Burley, usually infected by mechanical inoculation but occasionally with aphids. The virus was purified from this host by a modification of Damirdagh & Shepherd's (1970) procedure using borate instead of phosphate buffer throughout, and infectivity was confirmed by inoculation on Nicotiana sylvestris.

Year of Publication1971
JournalJournal of General Virology
Journal citation13 (Nov), pp. 357-359
Digital Object Identifier (DOI)doi:10.1099/0022-1317-13-2-357
Open accessPublished as bronze (free) open access
Publisher's version
Output statusPublished
Publication dates
Print01 Nov 1971
Publication process dates
Accepted16 Aug 1971
Copyright licensePublisher copyright
PublisherMicrobiology Society

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