Development of a reproducible method of analysis of iron, zinc and phosphorus in vegetables digests by SEC-ICP-MS

A - Papers appearing in refereed journals

Dellaquila, C., Neal, A. L. and Shewry, P. R. 2019. Development of a reproducible method of analysis of iron, zinc and phosphorus in vegetables digests by SEC-ICP-MS . Food Chemistry. 308 (2020), p. 125652.

AuthorsDellaquila, C., Neal, A. L. and Shewry, P. R.
Abstract

Vegetables contain iron, zinc and phosphorus as complexes with phytates limiting their availability from a vegetarian diet, meaning non-haem iron deficiency anaemia and zinc deficiency immune malfunction are a risk. Although these elements have been analysed previously in biological fluids and cereal using LC-ICP-MS, there is no method suitable for analysing iron, zinc and phosphorus simultaneously in vegetables because of their complex matrix. In this study, we analysed iron, zinc and phosphorus in cabbage, broccoli, pepper, spinach, kale and rocket after a simulated gastrointestinal digestion using a newly optimised SEC-ICP-MS method. Ammonium nitrate, as the mobile phase, and a suitable rinsing regime, allowed good reproducibility and maintenance of the equipment. The method showed good reproducibility and can be easily adapted to other vegetables, as required.

KeywordsSEC-ICP-MS; Method development; Elements; Vegetables
Year of Publication2019
JournalFood Chemistry
Journal citation308 (2020), p. 125652
Digital Object Identifier (DOI)doi:10.1016/j.foodchem.2019.125652
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeSpeciation and bioavailability of iron in plant foods
Publisher's version
Output statusPublished
Publication dates
Online15 Oct 2019
Publication process dates
Accepted05 Oct 2019
PublisherElsevier Sci Ltd
ISSN0308-8146

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