Subcellular dynamics studies of iron reveal how tissue-specific distribution patterns are established in developing wheat grains

A - Papers appearing in refereed journals

Sheraz, S., Wan, Y., Venter, E., Verma, S. K., Xiong, Q., Waites, J., Connorton, J. M, Shewry, P. R., Moore, K. L., and Balk, J 2021. Subcellular dynamics studies of iron reveal how tissue-specific distribution patterns are established in developing wheat grains. New Phytologist. 231 (4), pp. 1644-1657. https://doi.org/10.1111/nph.17440

AuthorsSheraz, S., Wan, Y., Venter, E., Verma, S. K., Xiong, Q., Waites, J., Connorton, J. M, Shewry, P. R., Moore, K. L., and Balk, J
Abstract

Understanding the mechanisms of iron trafficking in plants is key to enhancing the nutritional quality of crops. Because it is difficult to image iron in transit, we currently have an incomplete picture of the route(s) of iron translocation in developing seeds and how the tissue-specific distribution is established.
We have used a novel approach, combining 57Fe isotope labelling and Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS), to visualize iron translocation between tissues and within cells in immature wheat grain, Triticum aestivum L.
This enabled us to track the main route of iron transport from maternal tissues to the embryo through the different cell types. Further evidence for this route was provided by genetically diverting iron into storage vacuoles, with confirmation provided by histological staining and TEM-EDS. Almost all iron in both control and transgenic grains was found in intracellular bodies, indicating symplastic rather than apoplastic transport. Furthermore, a new type of iron body, highly enriched in 57Fe, was observed in aleurone cells and may represent iron being delivered to phytate globoids.
Correlation of the 57Fe enrichment profiles obtained by NanoSIMS with tissue-specific gene expression provides an updated model of iron homeostasis in cereal grains with relevance for future biofortification strategies.

KeywordsAleurone; Iron; NanoSIMS; Nicotianamine; Pulse-chase; Trafficking; Wheat
Year of Publication2021
JournalNew Phytologist
Journal citation231 (4), pp. 1644-1657
Digital Object Identifier (DOI)https://doi.org/10.1111/nph.17440
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCROPNUT: increasing iron in cereals
Publisher's version
Accepted author manuscript
Output statusPublished
Publication dates
Online29 Apr 2021
Publication process dates
Accepted14 Apr 2021
PublisherWiley
ISSN0028-646X

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