Development of a method for the detection of zinc in Brassica oleracea using solid phase extraction and size-exclusion chromatography inductively coupled plasma mass spectrometry (SEC-ICP-MS)

A - Papers appearing in refereed journals

Caterina Dell’Aquila 2021. Development of a method for the detection of zinc in Brassica oleracea using solid phase extraction and size-exclusion chromatography inductively coupled plasma mass spectrometry (SEC-ICP-MS). MethodsX. 8, p. 101428. https://doi.org/10.1016/j.mex.2021.101428

AuthorsCaterina Dell’Aquila
Abstract

The aim of this work is the development of a suitable method to extract and detect zinc-bound compounds
from cabbage, broccoli and kale (family Brassicaceae, species oleracea) using solid phase extraction (SPE) and
size-exclusion chromatography inductively coupled plasma mass spectrometry (SEC-ICP-MS). Tris [2-Amino-2-
(hydroxymethyl)-1,3-propanediol]/hydrochloric acid (Tris/HCl) or ammonium nitrate were used as extractants
added to the freeze-dried samples, which were then sonicated and centrifuged. An enzymatic mixture was
added to the extracts and then incubated for 5- and 18 h prior to analysis by SEC-ICP-MS. Results showed a
good coefficient of variation (CV) of the elution time (0.06-0.9%), concentration (4.7–16.9%) and molecular size
(0.4–5.4%). The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.9 μg L−1 and 2.8 μg L−1,
respectively. The proposed method is reliable and robust and can be applied to samples with difficult matrices
like vegetables and soil.

KeywordsSEC-ICP-MS; Vegetables; Extraction; Zinc; Enzyme
Year of Publication2021
JournalMethodsX
Journal citation8, p. 101428
Digital Object Identifier (DOI)https://doi.org/10.1016/j.mex.2021.101428
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDRINC/L025515/1
Publisher's version
Output statusPublished
Publication dates
Online24 Jun 2021
Publication process dates
Accepted23 Jun 2021
ISSN1095-9130
PublisherElsevier

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