Defining Composition and Function of the Rhizosphere Microbiota of Barley Genotypes Exposed to Growth-Limiting Nitrogen Supplies

A - Papers appearing in refereed journals

Alegria-Terrazas, R., Robertson-Albertyn, S., Corral, A. M., Escudero-Martinez, C., Kapadia, R., Balbirnie-Cumming, K., Morris, J., Hedley, P. E., Barret, M., Torres-Cortes, G., Paterson, E., Baggs, E. M., Abbott, J. and Bulgarelli, D. 2022. Defining Composition and Function of the Rhizosphere Microbiota of Barley Genotypes Exposed to Growth-Limiting Nitrogen Supplies. mSystems. https://doi.org/10.1128/msystems.00934-22

AuthorsAlegria-Terrazas, R., Robertson-Albertyn, S., Corral, A. M., Escudero-Martinez, C., Kapadia, R., Balbirnie-Cumming, K., Morris, J., Hedley, P. E., Barret, M., Torres-Cortes, G., Paterson, E., Baggs, E. M., Abbott, J. and Bulgarelli, D.
Abstract

The microbiota populating the rhizosphere, the interface between roots and soil, can modulate plant growth, development, and health. These microbial communities are not stochastically assembled from the surrounding soil, but their composition and putative function are controlled, at least partially, by the host plant. Here, we use the staple cereal barley as a model to gain novel insights into the impact of differential applications of nitrogen, a rate-limiting step for global crop production, on the host genetic control of the rhizosphere microbiota. Using a high-throughput amplicon sequencing survey, we determined that nitrogen availability for plant uptake is a factor promoting the selective enrichment of individual taxa in the rhizosphere of wild and domesticated barley genotypes. Shotgun sequencing and metagenome-assembled genomes revealed that this taxonomic diversification is mirrored by a functional specialization, manifested by the differential enrichment of multiple Gene Ontology terms, of the microbiota of plants exposed to nitrogen conditions limiting barley growth. Finally, a plant soil feedback experiment revealed that host control of the barley microbiota underpins the assembly of a phylogenetically diverse group of bacteria putatively required to sustain plant performance under nitrogen-limiting supplies. Taken together, our observations indicate that under nitrogen conditions limiting plant growth, host-microbe and microbe-microbe interactions fine-tune the host genetic selection of the barley microbiota at both taxonomic and functional levels. The disruption of these recruitment cues negatively impacts plant growth.

KeywordsBarley; Metagenomics; Nitrogen; Rhizosphere-inhabiting microbes
Year of Publication2022
JournalmSystems
Digital Object Identifier (DOI)https://doi.org/10.1128/msystems.00934-22
Web address (URL)https://doi.org/10.1128/msystems.00934-22
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeBB/S002871/1
Publisher's version
Output statusPublished
Publication dates
Online07 Nov 2022
Publication process dates
Accepted28 Sep 2022
PublisherAmerican Society for Microbiology
ISSN2379-5077

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