Impact of mutations in the target-encoding CYP51 gene in Mycosphaerella graminicola populations developing resistance to triazole fungicides
Project (past)
| Project dates | 01 Dec 2007 to end of 01 May 2011 |
|---|---|
| Funder | Biotechnology and Biological Sciences Research Council |
| Project number | S4906 |
| 4906 | |
| Department | Plant Pathology and Microbiology (PPM) |
| Research fish code | BBS/E/C/00004906 |
| Grant ID | BBS/E/C/00004906 |
Outputs
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Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype.
A - Papers appearing in refereed journals
Cools, H. J., Bayon, C., Atkins, S., Lucas, J. A. and Fraaije, B. A. 2012. Overexpression of the sterol 14α-demethylase gene (MgCYP51) in Mycosphaerella graminicola isolates confers a novel azole fungicide sensitivity phenotype. Pest Management Science. 68 (7), pp. 1034-1040. https://doi.org/10.1002/ps.3263
Molecular modelling of the emergence of azole resistance in Mycosphaerella graminicola
A - Papers appearing in refereed journals
Mullins, J. G. L., Parker, J. E., Cools, H. J., Togawa, R. C., Lucas, J. A., Fraaije, B. A., Kelly, D. E. and Kelly, S. L. 2011. Molecular modelling of the emergence of azole resistance in Mycosphaerella graminicola. PLOS ONE. 6, p. e20973. https://doi.org/10.1371/journal.pone.0020973
Impact of recently emerged sterol 14alpha-demethylase (CYP51) variants of Mycosphaerella graminicola on azole fungicide sensitivity
A - Papers appearing in refereed journals
Cools, H. J., Mullins, J. G. L., Fraaije, B. A., Parker, J. E., Kelly, D. E., Lucas, J. A. and Kelly, S. L. 2011. Impact of recently emerged sterol 14alpha-demethylase (CYP51) variants of Mycosphaerella graminicola on azole fungicide sensitivity. Applied and Environmental Microbiology. 77, pp. 3830-3837. https://doi.org/10.1128/AEM.00027-11
Mechanism of binding of prothioconazole to Mycosphaerella graminicola CYP51 differs from that of other azole antifungals
A - Papers appearing in refereed journals
Parker, J. E., Warrilow, A. G. S., Cools, H. J., Martel, C. M., Nes, W. D., Fraaije, B. A., Lucas, J. A., Kelly, D. E. and Kelly, S. L. 2011. Mechanism of binding of prothioconazole to Mycosphaerella graminicola CYP51 differs from that of other azole antifungals. Applied and Environmental Microbiology. 77, pp. 1460-1465. https://doi.org/10.1128/AEM.01332-10
Prothioconazole binds to Mycosphaerella graminicola CYP51 by a different mechanism compared to other azole antifungals
C2 - Non-edited contributions to conferences
Parker, J. E., Warrilow, A., Cools, H. J., Martel, C. M., Nes, W. D., Fraaije, B. A., Lucas, J. A., Kelly, D. E. and Kelly, S. L. 2010. Prothioconazole binds to Mycosphaerella graminicola CYP51 by a different mechanism compared to other azole antifungals. Abstracts 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Woods Hole, 3-7 October 2010 .
Recent evolution of P450 sterol 14a-demethylase (CYP51) of the wheat pathogen Mycosphaerella graminicola in response to selection by azole fungicides
C2 - Non-edited contributions to conferences
Cools, H. J. 2010. Recent evolution of P450 sterol 14a-demethylase (CYP51) of the wheat pathogen Mycosphaerella graminicola in response to selection by azole fungicides. Abstracts 10th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Woods Hole, 3-7 October 2010 .
Heterologous expression of mutated eburicol 14alpha-demethylase (CYP51) proteins of Mycosphaerella graminicola to assess effects on azole fungicide sensitivity and intrinsic protein function
A - Papers appearing in refereed journals
Cools, H. J., Parker, J. E., Kelly, D. E., Lucas, J. A., Fraaije, B. A. and Kelly, S. L. 2010. Heterologous expression of mutated eburicol 14alpha-demethylase (CYP51) proteins of Mycosphaerella graminicola to assess effects on azole fungicide sensitivity and intrinsic protein function. Applied and Environmental Microbiology. 76, pp. 2866-2872. https://doi.org/10.1128/AEM.02158-09
Functional characterisation of Mycosphaerella graminicola sterol 14alpha-demethylase (MgCYP51) variants resistant to azole fungicides
C2 - Non-edited contributions to conferences
Cools, H. J., Parker, J. E., Motteram, J., Kelly, D. E., Lucas, J. A., Rudd, J. J., Kelly, S. L. and Fraaije, B. A. 2010. Functional characterisation of Mycosphaerella graminicola sterol 14alpha-demethylase (MgCYP51) variants resistant to azole fungicides. Abstracts 16th International Reinhardsbrunn Symposium: Modern Fungicides and Antifungal Compounds, Friedrichroda, 25-29 April 2010 .
Sterol content analysis suggests altered eburicol 14alpha-demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted to azole fungicides
A - Papers appearing in refereed journals
Bean, T. P., Cools, H. J., Lucas, J. A., Hawkins, N. D., Ward, J. L., Shaw, M. W. and Fraaije, B. A. 2009. Sterol content analysis suggests altered eburicol 14alpha-demethylase (CYP51) activity in isolates of Mycosphaerella graminicola adapted to azole fungicides. FEMS Microbiology Letters. 296, pp. 266-273. https://doi.org/10.1111/j.1574-6968.2009.01645.x
Characterisation of mechanisms correlated with reduced azole sensitivity in Mycosphaerella graminicola
C2 - Non-edited contributions to conferences
Cools, H. J., Fraaije, B. A., Bean, T. P. and Lucas, J. A. 2008. Characterisation of mechanisms correlated with reduced azole sensitivity in Mycosphaerella graminicola.
Impact of mutations in the CYP51 gene of Mycosphaerella graminicola on azole fungicide sensitivity and intrinsic protein function
C2 - Non-edited contributions to conferences
Cools, H. J., Fraaije, B. A., Fernandez-Ortuno, D., Bean, T. P. and Lucas, J. A. 2008. Impact of mutations in the CYP51 gene of Mycosphaerella graminicola on azole fungicide sensitivity and intrinsic protein function. Abstracts 7th International Mycosphaerella and Stagonospora Symposium, Ascona, 18-22 August 2008 .