An electrophysiological investigation of the susceptible (Cooper) and resistant (kdr; super-kdr ) strains of the adult housefly, Musca domestica L.

A - Papers appearing in refereed journals

Gibson, A. J., Osborne, M. P., Ross, H. F. and Sawicki, R. M. 1988. An electrophysiological investigation of the susceptible (Cooper) and resistant (kdr; super-kdr ) strains of the adult housefly, Musca domestica L. Pesticide Science. 23 (3), pp. 283-292. https://doi.org/10.1002/ps.2780230310

AuthorsGibson, A. J., Osborne, M. P., Ross, H. F. and Sawicki, R. M.
Abstract

Sensory fibres in the isolated metathoracic leg of houseflies were used as a neurophysiological assay to investigate nerve insensitivity factors in knockdown-resistant strains (kdr, super-kdr) against DDT, the pyrethroids deltamethrin and RU15525, and the N-alkyl amide BTG502. Several neurophysiological effects were induced by these compounds, but the only consistent one was an elevation of the spontaneously occurring firing rate. Whilst both resistant strains possess a nerve insensitivity factor, that of the more resistant super-kdr flies appeared to be weaker than that of kdr. When probed with BTG502, super-kdr nerves were no more resistant to this compound than those of the susceptible Cooper strain. Kdr nerves were considerably less sensitive (at least 100-fold) than either of these other two strains. These results indicate that the voltage-dependent sodium channels are different in all three strains and that the higher resistance shown by super-kdr over kdr flies cannot be accounted for by simple enhancement of the kdr nerve-insensitivity factor. Therefore, target sites other than the voltage-dependent sodium channel have to be considered to account fully for super-kdr resistance. Such sites could occur at synaptic contacts, possibly involving phosphorylation of proteins and/or calcium-ion regulation.

KeywordsAgronomy; Entomology
Year of Publication1988
JournalPesticide Science
Journal citation23 (3), pp. 283-292
Digital Object Identifier (DOI)https://doi.org/10.1002/ps.2780230310
Open accessPublished as non-open access
Funder project or code25
ISSN0031613X
PublisherWiley

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