Characterisation of a 34kD protein from potato cyst nematodes, using monoclonal antibodies with potential for species diagnosis

A - Papers appearing in refereed journals

Robinson, M. P., Butcher, G., Curtis, R. H., Davies, K. G. and Evans, K. 1993. Characterisation of a 34kD protein from potato cyst nematodes, using monoclonal antibodies with potential for species diagnosis. Annals of Applied Biology - AAB. 123 (2), pp. 337-347.

AuthorsRobinson, M. P., Butcher, G., Curtis, R. H., Davies, K. G. and Evans, K.
Abstract

Two monoclonal antibodies, which differentially recognise the two species of potato cyst nematodes (PCN), Globodera pallida and G. rostochiensis, are described. They have been shown to have potential for quantification of these two species, recognising proteins of the same molecular weight (34 kD) in both species. Further investigation showed these proteins to have isoelectric points at pH values of 5.7 in G. pallida and 5.9 in G. rostochiensis, in common with the proteins used by Fleming and Marks (1983) to differentiate the species of PCN. They are likely to be structurally very similar, with the same physiological function (and therefore similar concentrations) in the two species. In crossreactivity tests with a wide range of soil nematode species, the antibodies reacted strongly only with species of the genus Globodera, and thereby confirmed their potential as the basis of a quantitative immunoassay likely to be useful in management of PCN populations.

KeywordsAgriculture, Multidisciplinary
Year of Publication1993
JournalAnnals of Applied Biology - AAB
Journal citation123 (2), pp. 337-347
Digital Object Identifier (DOI)doi:10.1111/j.1744-7348.1993.tb04096.x
Open accessPublished as non-open access
Funder project or code916
106
209
Project: 041010
Project: 041329
ISSN00034746
PublisherWiley

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