A facile method for screening for phosphinothricin (PPT)-resistant transgenic wheats

A - Papers appearing in refereed journals

Rasco-Gaunt, S., Riley, A., Lazzeri, P. A. and Barcelo, P. 1999. A facile method for screening for phosphinothricin (PPT)-resistant transgenic wheats. Molecular Breeding. 5 (3), pp. 255-262. https://doi.org/10.1023/A:1009689906936

AuthorsRasco-Gaunt, S., Riley, A., Lazzeri, P. A. and Barcelo, P.
Abstract

A bioassay was developed for identifying transgenic wheat plants based on De Block et al.'s [8] ammonium-multiwell assay which allows qualitative and quantitative evaluation of the expression of the enzyme phosphinothricin acetyl transferase (PAT). Important parameters in the assay are the use of young leaf tissues, short incubation period (6 h) and a high light intensity during incubation (250 μmol s−1m−2). The assay is quick and results are obtained within a day. Ammonium measurements based on a colourimetric (modified Berthelot) reaction are conducted using sodium salicylate to avoid the use of phenol. Results of the assay show high correlation with Basta leaf painting tests and polymerase chain reaction (PCR) results. Thus, the assay may be used as a facile screen for bar-expressing transgenic cereals.

Year of Publication1999
JournalMolecular Breeding
Journal citation5 (3), pp. 255-262
Digital Object Identifier (DOI)https://doi.org/10.1023/A:1009689906936
Open accessPublished as non-open access
Funder project or code316
411
Project: 011579
Publisher's version
Copyright license
Publisher copyright
Output statusPublished
Publication dates
Online01 Oct 1999
Publication process dates
Accepted30 Nov 1998
ISSN1380-3743
PublisherSpringer

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