A facile method for screening for phosphinothricin (PPT)-resistant transgenic wheats
A - Papers appearing in refereed journals
Rasco-Gaunt, S., Riley, A., Lazzeri, P. A. and Barcelo, P. 1999. A facile method for screening for phosphinothricin (PPT)-resistant transgenic wheats. Molecular Breeding. 5 (3), pp. 255-262. https://doi.org/10.1023/A:1009689906936
| Authors | Rasco-Gaunt, S., Riley, A., Lazzeri, P. A. and Barcelo, P. |
|---|---|
| Abstract | A bioassay was developed for identifying transgenic wheat plants based on De Block et al.'s [8] ammonium-multiwell assay which allows qualitative and quantitative evaluation of the expression of the enzyme phosphinothricin acetyl transferase (PAT). Important parameters in the assay are the use of young leaf tissues, short incubation period (6 h) and a high light intensity during incubation (250 μmol s−1m−2). The assay is quick and results are obtained within a day. Ammonium measurements based on a colourimetric (modified Berthelot) reaction are conducted using sodium salicylate to avoid the use of phenol. Results of the assay show high correlation with Basta leaf painting tests and polymerase chain reaction (PCR) results. Thus, the assay may be used as a facile screen for bar-expressing transgenic cereals. |
| Year of Publication | 1999 |
| Journal | Molecular Breeding |
| Journal citation | 5 (3), pp. 255-262 |
| Digital Object Identifier (DOI) | https://doi.org/10.1023/A:1009689906936 |
| Open access | Published as non-open access |
| Funder project or code | 316 |
| 411 | |
| Project: 011579 | |
| Publisher's version | Copyright license Publisher copyright |
| Output status | Published |
| Publication dates | |
| Online | 01 Oct 1999 |
| Publication process dates | |
| Accepted | 30 Nov 1998 |
| ISSN | 1380-3743 |
| Publisher | Springer |
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