The first meiosis of resynthesized Brassica napus, a genome blender

A - Papers appearing in refereed journals

Szadkowski, E., Eber, F., Huteau, V., Lode, M., Huneau, C., Belcram, H., Coriton, O., Manzanares-Dauleux, M. J., Delourme, R., King, G. J., Chalhoub, B., Jenczewski, E. and Chevre, A-M. 2010. The first meiosis of resynthesized Brassica napus, a genome blender. New Phytologist. 186 (1), pp. 102-112. https://doi.org/10.1111/j.1469-8137.2010.03182.x

AuthorsSzadkowski, E., Eber, F., Huteau, V., Lode, M., Huneau, C., Belcram, H., Coriton, O., Manzanares-Dauleux, M. J., Delourme, R., King, G. J., Chalhoub, B., Jenczewski, E. and Chevre, A-M.
Abstract

P>Polyploidy promotes the restructuring of merged genomes within initial generations of resynthesized Brassica napus, possibly caused by homoeologous recombination at meiosis. However, little is known about the impact of the first confrontation of two genomes at the first meiosis which could lead to genome exchanges in progeny. Here, we assessed the role of the first meiosis in the genome instability of synthetic B. napus. We used three different newly resynthesized B. napus plants and established meiotic pairing frequencies for the A and C genomes. We genotyped the three corresponding progenies in a cross to a natural B. napus on the two homoeologous A1 and C1 chromosomes. Pairing at meiosis in a set of progenies with various rearrangements was scored. Here, we confirmed that the very first meiosis of resynthesized plants of B. napus acts as a genome blender, with many of the meiotic-driven genetic changes transmitted to the progenies, in proportions that depend significantly on the cytoplasm background inherited from the progenitors. We conclude that the first meiosis generates rearrangements on both genomes and promotes subsequent restructuring in further generations. Our study advances the knowledge on the timing of genetic changes and the mechanisms that may bias their transmission.

KeywordsPlant Sciences
Year of Publication2010
JournalNew Phytologist
Journal citation186 (1), pp. 102-112
Digital Object Identifier (DOI)https://doi.org/10.1111/j.1469-8137.2010.03182.x
PubMed ID20149113
Open accessPublished as non-open access
FunderFrench Research Ministry - MENRT
ANR - Agence Nationale de la Recherche - The French National Research Agency
Funder project or codeCentre for Crop Genetic Improvement (CGI)
Project: 4974
Publisher's version
PublisherWiley
Grant IDANR-05-BDIV-015
ISSN0028-646X

Permalink - https://repository.rothamsted.ac.uk/item/8q541/the-first-meiosis-of-resynthesized-brassica-napus-a-genome-blender

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