The CACTA transposon Bot1 played a major role in Brassica genome divergence and gene proliferation

A - Papers appearing in refereed journals

Alix, K., Joets, J., Ryder, C. D., Moore, J., Barker, G. C., Bailey, J. P., King, G. J. and Heslop-Harrison, J. S. 2008. The CACTA transposon Bot1 played a major role in Brassica genome divergence and gene proliferation. The Plant Journal. 56 (6), pp. 1030-1044. https://doi.org/10.1111/j.1365-313X.2008.03660.x

AuthorsAlix, K., Joets, J., Ryder, C. D., Moore, J., Barker, G. C., Bailey, J. P., King, G. J. and Heslop-Harrison, J. S.
Abstract

We isolated and characterized a Brassica C genome-specific CACTA element, which was designated Bot1 (Brassica oleracea transposon 1). After analysing phylogenetic relationships, copy numbers and sequence similarity of Bot1 and Bot1 analogues in B. oleracea (C genome) versus Brassica rapa (A genome), we concluded that Bot1 has encountered several rounds of amplification in the oleracea genome only, and has played a major role in the recent rapa and oleracea genome divergence. We performed in silico analyses of the genomic organization and internal structure of Bot1, and established which segment of Bot1 is C-genome specific. Our work reports a fully characterized Brassica repetitive sequence that can distinguish the Brassica A and C chromosomes in the allotetraploid Brassica napus, by fluorescent in situ hybridization. We demonstrated that Bot1 carries a host S locus-associated SLL3 gene copy. We speculate that Bot1 was involved in the proliferation of SLL3 around the Brassica genome. The present study reinforces the assumption that transposons are a major driver of genome and gene evolution in higher plants.

KeywordsPlant Sciences
Year of Publication2008
JournalThe Plant Journal
Journal citation56 (6), pp. 1030-1044
Digital Object Identifier (DOI)https://doi.org/10.1111/j.1365-313X.2008.03660.x
PubMed ID18764926
Open accessPublished as non-open access
FunderNational Science Foundation - US
Funder project or codeCentre for Crop Genetic Improvement (CGI)
Project: 4719
ISSN09607412
0960-7412
PublisherWiley

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