Generation of nonvernal-obligate, faster-cycling Noccaea caerulescens lines through fast neutron mutagenesis

A - Papers appearing in refereed journals

O Lochlainn, S., Fray, R. G., Hammond, J. P., King, G. J., White, P. J., Young, S. D. and Broadley, M. R. 2011. Generation of nonvernal-obligate, faster-cycling Noccaea caerulescens lines through fast neutron mutagenesis. New Phytologist. 189 (2), pp. 409-414.

AuthorsO Lochlainn, S., Fray, R. G., Hammond, J. P., King, G. J., White, P. J., Young, S. D. and Broadley, M. R.
Abstract

P>Noccaea caerulescens (formerly Thlaspi caerulescens) is a widely studied metal hyperaccumulator. However, molecular genetic studies are challenging in this species because of its vernal-obligate biennial life cycle of 7-9 months. Here, we describe the development of genetically stable, faster cycling lines of N. caerulescens which are nonvernal-obligate. A total of 5500 M-0 seeds from Saint Laurent Le Minier (France) were subjected to fast neutron mutagenesis. Following vernalization of young plants, 79% of plants survived to maturity. In all, 80 000 M-2 lines were screened for flowering in the absence of vernalization. Floral initials were observed in 35 lines, with nine flowering in < 12 wk. Two lines (A2 and A7) were selfed to the M-4 generation. Floral initials were observed 66 and 87 d after sowing (DAS) in A2 and A7, respectively. Silicle development occurred for all A2 and for most A7 at 92 and 123 DAS, respectively. Floral or silicle development was not observed in wild-type (WT) plants. Leaf zinc (Zn) concentration was similar in WT, A2 and A7 lines. These lines should facilitate future genetic studies of this remarkable species. Seed is publicly available through the European Arabidopsis Stock Centre (NASC).

KeywordsPlant Sciences
Year of Publication2011
JournalNew Phytologist
Journal citation189 (2), pp. 409-414
Digital Object Identifier (DOI)doi:10.1111/j.1469-8137.2010.03554.x
PubMed ID21058953
Open accessPublished as green open access
FunderScottish Government's Rural and Environment Science and Analytical Services Division (RESAS)
Biotechnology and Biological Sciences Research Council
Funder project or codeCentre for Crop Genetic Improvement (CGI)
Project: 5034
Publisher's version
PublisherWiley
Grant IDBBSSE200613215
ISSN0028-646X

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