O-GlcNAcylation of master growth repressor DELLA by SECRET AGENT modulates multiple signaling pathways in Arabidopsis

A - Papers appearing in refereed journals

Zentella, R., Hu, J. H., Hsieh, W. P., Matsumoto, P. A., Dawdy, A., Barnhill, B., Oldenhof, H., Hartweck, L. M., Maitra, S., Thomas, S. G., Cockrell, S., Boyce, M., Shabanowitz, J., Hunt, D. F., Olszewski, N. E. and Sun, T. P. 2016. O-GlcNAcylation of master growth repressor DELLA by SECRET AGENT modulates multiple signaling pathways in Arabidopsis. Genes & Development. 30 (2), pp. 164-176. https://doi.org/10.1101/gad.270587.115

AuthorsZentella, R., Hu, J. H., Hsieh, W. P., Matsumoto, P. A., Dawdy, A., Barnhill, B., Oldenhof, H., Hartweck, L. M., Maitra, S., Thomas, S. G., Cockrell, S., Boyce, M., Shabanowitz, J., Hunt, D. F., Olszewski, N. E. and Sun, T. P.
Abstract

The DELLA family of transcription regulators functions as master growth repressors in plants by inhibiting phytohormone gibberellin (GA) signaling in response to developmental and environmental cues. DELLAs also play a central role in mediating cross-talk between GA and other signaling pathways via antagonistic direct interactions with key transcription factors. However, how these crucial protein–protein interactions can be dynamically regulated during plant development remains unclear. Here, we show that DELLAs are modified by the O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) SECRET AGENT (SEC) in Arabidopsis. O-GlcNAcylation of the DELLA protein REPRESSOR OF ga1-3 (RGA) inhibits RGA binding to four of its interactors—PHYTOCHROME-INTERACTING FACTOR3 (PIF3), PIF4, JASMONATE-ZIM DOMAIN1, and BRASSINAZOLE-RESISTANT1 (BZR1)—that are key regulators in light, jasmonate, and brassinosteroid signaling pathways, respectively. Consistent with this, the sec-null mutant displayed reduced responses to GA and brassinosteroid and showed decreased expression of several common target genes of DELLAs, BZR1, and PIFs. Our results reveal a direct role of OGT in repressing DELLA activity and indicate that O-GlcNAcylation of DELLAs provides a fine-tuning mechanism in coordinating multiple signaling activities during plant development.

KeywordsO-GlcNAcylation of DELLA; DELLA-interacting proteins; hormone signaling; gibberellin signaling; O-GlcNAc transferase; SECRET AGENT
Year of Publication2016
JournalGenes & Development
Journal citation30 (2), pp. 164-176
Digital Object Identifier (DOI)https://doi.org/10.1101/gad.270587.115
PubMed ID26773002
PubMed Central IDPMC4719307
Web address (URL)http://genesdev.cshlp.org/content/30/2/164.full
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeWheat
[20:20 Wheat] Maximising yield potential of wheat
Publisher's version
Output statusPublished
Publication dates
Online30 Nov 2015
Publication process dates
Accepted30 Nov 2015
Copyright licenseCC BY-NC
PublisherCold Spring Harbor Lab Press, Publications Dept
ISSN0890-9369

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