A - Papers appearing in refereed journals
Sigalas, P., Buchner, P. H., Thomas, S. G., Jamois, F., Arkourn, M., Yvin, J.- C., Bennett, M. J. and Hawkesford, M. J. 2023. Nutritional and tissue-specific regulation of cytochrome P450 CYP711A MAX1 homologues and strigolactone biosynthesis in wheat. Journal of Experimental Botany. 74 (6), pp. 1890-1910. https://doi.org/10.1093/jxb/erad008
Authors | Sigalas, P., Buchner, P. H., Thomas, S. G., Jamois, F., Arkourn, M., Yvin, J.- C., Bennett, M. J. and Hawkesford, M. J. |
---|---|
Abstract | Strigolactones (SLs) are a class of phytohormones regulating branching/tillering, and their biosynthesis has been associated with nutritional signals and plant adaptation to nutrient-limiting conditions. The enzymes in the SL biosynthetic pathway downstream of carlactone are of interest as they are responsible for structural diversity in SLs, particularly cytochrome P450 CYP711A subfamily members, such as MORE AXILLARY GROWTH1 (MAX1) in Arabidopsis. We identified 13 MAX1 homologues in wheat, clustering in four clades and five homoeologous subgroups. The utilization of RNA-sequencing data revealed a distinct expression pattern of MAX1 homologues in above- and below-ground tissues, providing insights into the distinct roles of MAX1 homologues in wheat. In addition, a transcriptional analysis showed that SL biosynthetic genes were systematically regulated by nitrogen supply. Nitrogen limitation led to larger transcriptional changes in the basal nodes than phosphorus limitation, which was consistent with the observed tillering suppression, as wheat showed higher sensitivity to nitrogen. The opposite was observed in roots, with phosphorus limitation leading to stronger induction of most SL biosynthetic genes compared with nitrogen limitation. The observed tissue-specific regulation of SL biosynthetic genes in response to nutritional signals is likely to reflect the dual role of SLs as rhizosphere signals and branching inhibitors. |
Keywords | Cytochrome P450 CYP711A tillering; Gene expression; MAX1; Nitrogen; Strigolactones; Tillering; Wheat (Triticum aestivum) |
Year of Publication | 2023 |
Journal | Journal of Experimental Botany |
Journal citation | 74 (6), pp. 1890-1910 |
Digital Object Identifier (DOI) | https://doi.org/10.1093/jxb/erad008 |
Open access | Published as ‘gold’ (paid) open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Designing Future Wheat - WP1 - Increased efficiency and sustainability |
Genetic variation and chemical control of tillering in wheat | |
Publisher's version | |
Output status | Published |
Publication dates | |
Online | 10 Jan 2023 |
Publication process dates | |
Accepted | 09 Jan 2023 |
Publisher | Oxford University Press (OUP) |
ISSN | 0022-0957 |
Permalink - https://repository.rothamsted.ac.uk/item/98v6x/nutritional-and-tissue-specific-regulation-of-cytochrome-p450-cyp711a-max1-homologues-and-strigolactone-biosynthesis-in-wheat