Localisation of iron and zinc in grain of biofortified wheat

A - Papers appearing in refereed journals

Wan, Y., Stewart, T., Amrahli, M., Evans, J., Sharp, P., Govindan, V., Hawkesford, M. J. and Shewry, P. R. 2022. Localisation of iron and zinc in grain of biofortified wheat. Journal of Cereal Science. 105 (May 22), p. 103470. https://doi.org/10.1016/j.jcs.2022.103470

AuthorsWan, Y., Stewart, T., Amrahli, M., Evans, J., Sharp, P., Govindan, V., Hawkesford, M. J. and Shewry, P. R.
Abstract

The dietary contributions of iron (Fe) and zinc (Zn) from cereals are determined by concentrations, locations and chemical forms. A genetically biofortified wheat line showed higher concentrations of Zn and Fe than three control lines when grown over two years. The mineral distributions determined using imaging (histochemical staining and LA-ICP-MS), sequential pearling and hand dissection showed no consistent differences between the two lines. Fe was most abundant in the aleurone layer and the scutellum and Zn in the scutellar epithelium, the endosperm transfer cells and embryonic axis. Pearling fractions showed positive correlations between the concentration of P and those of Zn and Fe in all fractions except the outermost layer. This is consistent with Fe and Zn being concentrated in phytates. Developing grains showed decreasing gradients in concentration from the proximal to the distal ends. The concentrations of Fe and Zn were therefore higher in the biofortified line than the control lines but their locations did not differ.

KeywordsWheat grain; Biofortification; Micronutrients; LA-ICP; MS; Localisation; Zinc; Iron
Year of Publication2022
JournalJournal of Cereal Science
Journal citation105 (May 22), p. 103470
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jcs.2022.103470
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
Publisher's version
Output statusPublished
Publication dates
Online03 Apr 2022
Publication process dates
Accepted25 Mar 2022
PublisherAcademic Press Ltd- Elsevier Science Ltd
ISSN0733-5210

Permalink - https://repository.rothamsted.ac.uk/item/98850/localisation-of-iron-and-zinc-in-grain-of-biofortified-wheat

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