Gradients of Gluten Proteins and Free Amino Acids along the Longitudinal Axis of the Developing Caryopsis of Bread Wheat

A - Papers appearing in refereed journals

Shi, Z., Wang, Y., Wan, Y., Hassall, K. L., Jiang, D., Shewry, P. R. and Hawkesford, M. J. 2019. Gradients of Gluten Proteins and Free Amino Acids along the Longitudinal Axis of the Developing Caryopsis of Bread Wheat . Journal of Agricultural and Food Chemistry. 67 (31), pp. 8706-8714.

AuthorsShi, Z., Wang, Y., Wan, Y., Hassall, K. L., Jiang, D., Shewry, P. R. and Hawkesford, M. J.
Abstract

Gradients in the contents and compositions of gluten proteins and free amino acids and the expression levels of gluten protein genes in developing wheat caryopses were determined by dividing the caryopsis into three longitudinal sections, namely, proximal (En1), middle (En2), and distal (En3) to embryo. The total gluten protein content was lower in En1 than in En2 and En3, with decreasing proportions of HMW-GS, LMW GS, and α/β- and γ-gliadins and increasing proportions of ωgliadins. These differences were associated with the abundances of gluten protein transcripts. Gradients in the proportions of the gluten protein polymers which affect dough processing quality also occurred, but not in total free amino acids. Microscopy showed that the lower gluten protein content in En1 may have resulted, at least in part, from the presence of modified cells in the dorsal part of En1, but the reasons for the differences in composition are not known.

KeywordsWheat grain; Gluten proteins; Free amino acids; Longitudinal gradients
Year of Publication2019
JournalJournal of Agricultural and Food Chemistry
Journal citation67 (31), pp. 8706-8714
Digital Object Identifier (DOI)doi:10.1021/acs.jafc.9b02728
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Agriculture and Horticulture Development Board
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
Developing novel types of low protein wheat for breadmaking
Publisher's version
Output statusPublished
Publication dates
Online16 Jul 2019
Publication process dates
Accepted16 Jul 2019
PublisherAmerican Chemical Society (ACS)
ISSN0021-8561

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