Using NMR-based metabolomics to evaluate postprandial urinary responses following consumption of minimally processed wheat bran or wheat aleurone by men and women

A - Papers appearing in refereed journals

Garg, R., Brennan, L., Price, R. K., Wallace, J. M. W., Strain, J. J., Gibney, M. J., Shewry, P. R., Ward, J. L., Garg, L. and Welch, R. W. 2016. Using NMR-based metabolomics to evaluate postprandial urinary responses following consumption of minimally processed wheat bran or wheat aleurone by men and women. Nutrients. 8 (2), p. 96.

AuthorsGarg, R., Brennan, L., Price, R. K., Wallace, J. M. W., Strain, J. J., Gibney, M. J., Shewry, P. R., Ward, J. L., Garg, L. and Welch, R. W.
Abstract

Wheat bran, and especially wheat aleurone fraction, are concentrated sources of a wide range of components which may contribute to the health benefits associated with higher consumption of whole-grain foods. This study used NMR metabolomics to evaluate urine samples from baseline at one and two hours postprandially, following the consumption of minimally processed bran, aleurone or control by 14 participants (7 Females; 7 Males) in a randomized crossover trial. The methodology discriminated between the urinary responses of control, and bran and aleurone, but not between the two fractions. Compared to control, consumption of aleurone or bran led to significantly and substantially higher urinary concentrations of lactate, alanine, N-acetylaspartate acid and N-acetylaspartylglutamate and significantly and substantially lower urinary betaine concentrations at one and two hours postprandially. There were sex related differences in urinary metabolite profiles with generally higher hippurate and citrate and lower betaine in females compared to males. Overall, this postprandial study suggests that acute consumption of bran or aleurone is associated with a number of physiological effects that may impact on energy metabolism and which are consistent with longer term human and animal metabolomic studies that used whole-grain wheat diets or wheat fractions

KeywordsNMR metabolomics; wheat bran; wheat aleurone; energy metabolism; sex differences; urine; lactate; alanine; N-acetylaspartate; N-acetylaspartylglutamate; betaine; hippurate
Year of Publication2016
JournalNutrients
Journal citation8 (2), p. 96
Digital Object Identifier (DOI)doi:10.3390/nu8020096
Open accessPublished as ‘gold’ (paid) open access
Funder project or codeDesigning Seeds for Nutrition and Health (DS)
Design Cereal Seeds [Shewry/Mitchell]
Publisher's version
Output statusPublished
Publication dates
Online17 Feb 2016
Publication process dates
Accepted04 Feb 2016
PublisherMDPI
MDPI
Copyright licenseCC BY
ISSN2072-6643

Permalink - https://repository.rothamsted.ac.uk/item/8v1z4/using-nmr-based-metabolomics-to-evaluate-postprandial-urinary-responses-following-consumption-of-minimally-processed-wheat-bran-or-wheat-aleurone-by-men-and-women

28 total views
4 total downloads
5 views this month
0 downloads this month
Download files as zip