A - Papers appearing in refereed journals
Rigby, N. M., Marsh, J., Sancho, A. I., Wellner, K., Akkerdaas, J., Van Ree, R., Knulst, A., Fernandez-Rivas, M., Brettlova, V., Schilte, P. P., Summer, C., Pumphrey, R., Shewry, P. R. and Mills, E. N. C. 2008. The purification and characterisation of allergenic hazelnut seed proteins. Molecular Nutrition & Food Research. 52, pp. S251-S261.
|Authors||Rigby, N. M., Marsh, J., Sancho, A. I., Wellner, K., Akkerdaas, J., Van Ree, R., Knulst, A., Fernandez-Rivas, M., Brettlova, V., Schilte, P. P., Summer, C., Pumphrey, R., Shewry, P. R. and Mills, E. N. C.|
A lipid transfer protein (LTP, Cor a 8) together with the 11S (Cor a 9) and 7S seed storage globulins (Cor a 11) are major food allergens present in hazelnut. Methods are described for their purification and characterisation using in-gel tryptic digestion mass spectrometry to confirm their identities and circular dichroism and Fourier-transform infrared spectroscopies to demonstrate that they are authentically folded. Preliminary immunochemical studies have also confirmed that the purified preparations retain their immunological properties in terms of immunoglobulin E binding, determined by immunoblotting using serum from hazelnut allergic patients. These preparations form a basis for development of improved methods of diagnosis of food allergy based on the concept of component-resolved diagnosis.
|Keywords||Food Science & Technology|
|Year of Publication||2008|
|Journal||Molecular Nutrition & Food Research|
|Journal citation||52, pp. S251-S261|
|Digital Object Identifier (DOI)||doi:10.1002/mnfr.200800083|
|Open access||Published as non-open access|
|Funder project or code||CGI|
|Funder||European Commission - EC|
|Biotechnology and Biological Sciences Research Council|
Permalink - https://repository.rothamsted.ac.uk/item/8q21y/the-purification-and-characterisation-of-allergenic-hazelnut-seed-proteins