The purification and characterisation of allergenic hazelnut seed proteins

A - Papers appearing in refereed journals

Rigby, N. M., Marsh, J., Sancho, A. I., Wellner, K., Akkerdaas, J., Van Ree, R., Knulst, A., Fernandez-Rivas, M., Brettlova, V., Schilte, P. P., Summer, C., Pumphrey, R., Shewry, P. R. and Mills, E. N. C. 2008. The purification and characterisation of allergenic hazelnut seed proteins. Molecular Nutrition & Food Research. 52, pp. S251-S261.

AuthorsRigby, N. M., Marsh, J., Sancho, A. I., Wellner, K., Akkerdaas, J., Van Ree, R., Knulst, A., Fernandez-Rivas, M., Brettlova, V., Schilte, P. P., Summer, C., Pumphrey, R., Shewry, P. R. and Mills, E. N. C.
Abstract

A lipid transfer protein (LTP, Cor a 8) together with the 11S (Cor a 9) and 7S seed storage globulins (Cor a 11) are major food allergens present in hazelnut. Methods are described for their purification and characterisation using in-gel tryptic digestion mass spectrometry to confirm their identities and circular dichroism and Fourier-transform infrared spectroscopies to demonstrate that they are authentically folded. Preliminary immunochemical studies have also confirmed that the purified preparations retain their immunological properties in terms of immunoglobulin E binding, determined by immunoblotting using serum from hazelnut allergic patients. These preparations form a basis for development of improved methods of diagnosis of food allergy based on the concept of component-resolved diagnosis.

KeywordsFood Science & Technology
Year of Publication2008
JournalMolecular Nutrition & Food Research
Journal citation52, pp. S251-S261
Digital Object Identifier (DOI)doi:10.1002/mnfr.200800083
PubMed ID19006093
Open accessPublished as non-open access
FunderEuropean Commission - EC
Biotechnology and Biological Sciences Research Council
Funder project or codeCentre for Crop Genetic Improvement (CGI)
ISSN16134125
PublisherWiley

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