Influence of temperature during grain filling on gluten viscoelastic properties and gluten protein composition

A - Papers appearing in refereed journals

Koga, S., Bocker, U., Moldestad, A., Tosi, P., Shewry, P. R., Mosleth, E. F. and Uhlen, A. K. 2016. Influence of temperature during grain filling on gluten viscoelastic properties and gluten protein composition. Journal of the Science of Food and Agriculture. 96 (1), pp. 122-130.

AuthorsKoga, S., Bocker, U., Moldestad, A., Tosi, P., Shewry, P. R., Mosleth, E. F. and Uhlen, A. K.
Abstract

BACKGROUND: The aim of this study was to investigate the effects of low to moderate temperatures on gluten functionality and gluten protein composition. Four spring wheat cultivars were grown in climate chambers with three temperature regimes (day/night temperatures of 13/10, 18/15 and 23/20 degrees C) during grain filling. RESULTS: The temperature strongly influenced grain weight and protein content. Gluten quality measured by maximum resistance to extension (R-max) was highest in three cultivars grown at 13 degrees C. R-max was positively correlated with the proportion of sodium dodecyl sulfate-unextractable polymeric proteins (%UPP). The proportions of omega-gliadins and D-type low-molecular-weight glutenin subunits (LMW-GS) increased and the proportions of alpha- and gamma-gliadins and B-type LMW-GS decreased with higher temperature, while the proportion of high-molecular-weight glutenin subunits (HMW-GS) was constant between temperatures. The cultivar Berserk had strong and constant R-max between the different temperatures. CONCLUSION: Constant low temperature, even as low as 13 degrees C, had no negative effects on gluten quality. The observed variation in R-max related to temperature could be explained more by %UPP than by changes in the proportions of HMW-GS or other gluten proteins. The four cultivars responded differently to temperature, as gluten from Berserk was stronger and more stable over a wide range of temperatures. (C) 2015 Society of Chemical Industry

KeywordsAgriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology
Year of Publication2016
JournalJournal of the Science of Food and Agriculture
Journal citation96 (1), pp. 122-130
Digital Object Identifier (DOI)doi:10.1002/jsfa.7068
PubMed ID25565275
Open accessPublished as non-open access
FunderFoundation for Research Levy on Agricultural Products (FFL)
PhD education program at Norwegian University of Life Sciences
Agricultural Agreement (JA)
Funder project or codeDesigning Seeds for Nutrition and Health (DS)
ISSN00225142
0022-5142
PublisherWiley
Grant ID190491

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