A - Papers appearing in refereed journals
Wilkinson, M. D., Tosi, P., Lovegrove, A., Corol, D. I., Ward, J. L., Palmer, R., Powers, S. J., Passmore, D., Webster, G., Marcus, S. E., Knox, J. P. and Shewry, P. R. 2017. The Gsp-1 genes encode the wheat arabinogalactan peptide. Journal of Cereal Science. 74 (March), pp. 155-164. https://doi.org/10.1016/j.jcs.2017.02.006
Authors | Wilkinson, M. D., Tosi, P., Lovegrove, A., Corol, D. I., Ward, J. L., Palmer, R., Powers, S. J., Passmore, D., Webster, G., Marcus, S. E., Knox, J. P. and Shewry, P. R. |
---|---|
Abstract | Western blotting, ELISA and 1H-NMR spectroscopy showed that RNAi down-regulation of the wheat Gsp-1 gene resulted in reduced contents of both arabinogalactan peptide (AGP) and grain softness protein (GSP-1) in mature wheat grains confirming that these components are encoded by the same gene. A small increase in grain hardness and decrease in the viscosity of aqueous extracts of the transgenic lines also indicated small effects on functional properties. Immunolocalisation using a novel wheat AGP monoclonal antibody in conjunction with confocal microscopy showed that the major form of AGP which was eliminated in knockout lines is located within the cell, probably in the vacuole, and not in the plasma membrane or cell wall. However, clear localisation of the AGP epitope to the plasma membrane was observed in both control and transgenic lines and probably resulted from the presence of one or more separate forms of arabinogalactan protein. The existence of such additional form(s) was also indicated by 1H-NMR spectroscopy which showed that the ratio of arabinose to galactose differed between the control and transgenic lines. |
Keywords | wheat; Arabinogalactan peptide; grain softness protein; immunocytochemistry |
Year of Publication | 2017 |
Journal | Journal of Cereal Science |
Journal citation | 74 (March), pp. 155-164 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.jcs.2017.02.006 |
Open access | Published as ‘gold’ (paid) open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Designing Seeds for Nutrition and Health (DS) |
Design Cereal Seeds [Shewry/Mitchell] | |
Publisher's version | Copyright license CC BY |
Output status | Published |
Publication dates | |
Online | 16 Feb 2017 |
Publication process dates | |
Accepted | 14 Feb 2017 |
Copyright license | CC BY |
ISSN | 0733-5210 |
Publisher | Academic Press Ltd- Elsevier Science Ltd |
Permalink - https://repository.rothamsted.ac.uk/item/8v42z/the-gsp-1-genes-encode-the-wheat-arabinogalactan-peptide