Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein-kinase cDNA

A - Papers appearing in refereed journals

Alderson, A., Sabelli, P. A., Dickinson, J. R., Cole, D., Richardson, M., Kreis, M., Shewry, P. R. and Halford, N. G. 1991. Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein-kinase cDNA. Proceedings of the National Academy of Sciences of the United States of America - PNAS. 88 (19), pp. 8602-8605.

AuthorsAlderson, A., Sabelli, P. A., Dickinson, J. R., Cole, D., Richardson, M., Kreis, M., Shewry, P. R. and Halford, N. G.
Abstract

A cDNA, cRKIN1, encoding a putative homologue of the yeast (Saccharomyces cerevisiae) SNF1-encoded protein-serine/threonine kinase, has been isolated from a library prepared from rye endosperm mRNA. Northern blot analysis demonstrated the presence of cRKIN1-related transcripts in developing endosperms but not in shoots, and Southern blot analysis showed the presence of a small gene family. SNF1 plays a central role in carbon catabolite repression in yeast and expression of the RKIN1 sequence in yeast snf1 mutants restored SNF1 function. This suggests that the RKIN1 protein has a role in the control of carbon metabolism in endosperms of rye. 

KeywordsRRES175; 175_Genetics; 175_Plant sciences; 175_Fungi; 175_Food science
Year of Publication1991
JournalProceedings of the National Academy of Sciences of the United States of America - PNAS
Journal citation88 (19), pp. 8602-8605
Digital Object Identifier (DOI)doi:10.1073/pnas.88.19.8602
PubMed ID1924320
Open accessPublished as non-open access
PublisherNational Academy of Sciences
National Academy of Sciences of the United States of America
ISSN0027-8424

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