Tobacco plants transformed with cdc25, a mitotic inducer gene from fission yeast

A - Papers appearing in refereed journals

Bell, M. H., Halford, N. G., Ormrod, J. C. and Francis, D. 1993. Tobacco plants transformed with cdc25, a mitotic inducer gene from fission yeast. Plant Molecular Biology. 23 (3), pp. 445-451.

AuthorsBell, M. H., Halford, N. G., Ormrod, J. C. and Francis, D.
Abstract

We investigated the effects of expressing a cDNA of cdc25, a mitotic inducer gene of Schizosaccharomyces pombe, on the development of transgenic tobacco plants (Nicotiana tabacum cv. Samsun). Nine independent primary transformants were regenerated containing the cdc25 sequence under the control of a cauliflower mosaic virus 35S gene promoter. Eight of the nine plants showed altered leaf morphology, the lamina being lengthened and twisted and the interveinal regions being pocketed. One of these was sacrificed for analysis of the root meristem, where the cells were found to be significantly smaller than in the wild type. The other seven were grown on and showed precocious flowering, flowers being produced earlier and in significantly greater numbers than in the wild type. They also developed abnormal flowers on short stalks developing in a position normally occupied by the most proximal axillary bud of otherwise normal flower pedicels. The presence or absence of these phenotypes in the primary transformants and in the T2 generation was associated with the presence or absence of detectable levels of cdc25 transcripts.

Keywordscdc25; Cell Cycle; mitotic inducer; Plant Development; transgenic; Tobacco; p34cdc2 protein-kinase; cell-cycle; tyrosine phosphorylation; arabidopsis-thaliana; m-phase; Sequences; Agrobacterium; division; Vectors; homolog
Year of Publication1993
JournalPlant Molecular Biology
Journal citation23 (3), pp. 445-451
Digital Object Identifier (DOI)doi:10.1007/bf00019293
Open accessPublished as non-open access
ISSN01674412

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