Reference gene selection for quantitative RT-PCR normalisation in barley under low nitrogen

A - Papers appearing in refereed journals

Chen, Z., Li, Y., Liu, C., Wang, Y., He, T., Guo, G., Fang, C., Gao, R., Xu, H., Zhou, L., Huang, J., Halford, N. G. and Lu, R. 2018. Reference gene selection for quantitative RT-PCR normalisation in barley under low nitrogen. Journal of Cereal Science. 82 (July), pp. 213-215.

AuthorsChen, Z., Li, Y., Liu, C., Wang, Y., He, T., Guo, G., Fang, C., Gao, R., Xu, H., Zhou, L., Huang, J., Halford, N. G. and Lu, R.
Abstract

The aim of the present study was to identify genes that could be used for the normalisation of qRT-PCR data in barley, using RNAseq data (NCBI accession number PRJNA400519: RNA sequencing of Hordeum vulgare L. under low nitrogen stress). The particular interest was in genes that were stably expressed under different nitrogen conditions, including low-nitrogen stress.

Keywordsbarley; reference gene; qRT-PCR; low-nitrogen stress
Year of Publication2018
JournalJournal of Cereal Science
Journal citation82 (July), pp. 213-215
Digital Object Identifier (DOI)doi:10.1016/j.jcs.2018.06.014
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDFW - Designing Future Wheat - Work package 1 (WP1) - Increased efficiency and sustainability
Publisher's version
Output statusPublished
Publication dates
Online27 Jun 2018
Publication process dates
Accepted26 Jun 2018
PublisherAcademic Press Ltd- Elsevier Science Ltd
Copyright licenseCC BY
ISSN0733-5210

Permalink - https://repository.rothamsted.ac.uk/item/847yv/reference-gene-selection-for-quantitative-rt-pcr-normalisation-in-barley-under-low-nitrogen

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