Genetic control of grain amino acid composition in a UK soft wheat mapping population

A - Papers appearing in refereed journals

Oddy, J., Chhetry, M., Awal, R., Addy, J., Wilkinson, M. D., Smith, D., King, R., Hall, C., Testa, R., Murray, E., Raffan, S., Curtis, T. Y., Wingen, L., Griffiths, S., Berry, S., Elmore, S. J., Cryer, N., Moreira de Almeida, I. and Halford, N. G. 2023. Genetic control of grain amino acid composition in a UK soft wheat mapping population. The Plant Genome. 16 (4), p. e20335. https://doi.org/10.1002/tpg2.20335

AuthorsOddy, J., Chhetry, M., Awal, R., Addy, J., Wilkinson, M. D., Smith, D., King, R., Hall, C., Testa, R., Murray, E., Raffan, S., Curtis, T. Y., Wingen, L., Griffiths, S., Berry, S., Elmore, S. J., Cryer, N., Moreira de Almeida, I. and Halford, N. G.
Abstract

Wheat is a major source of nutrients for populations across the globe, but the amino acid composition of wheat grain does not provide optimal nutrition. The nutritional value of wheat grain is limited by low concentrations of lysine (the most limiting essential amino acid) and high concentrations of free asparagine (precursor to the processing contaminant acrylamide). There are currently few available solutions for asparagine reduction and lysine biofortification through breeding. In this study, we investigated the genetic architecture controlling grain free amino acid composition and its relationship to other traits in a Robigus × Claire doubled haploid population. Multivariate analysis of amino acids and other quality traits showed that the two groups are largely independent of one another, with the largest effect on amino acids being from the environment. Linkage analysis of the population allowed identification of QTL controlling free amino acids and quality traits, and this was compared against genomic prediction methods. Following identification of a QTL controlling free lysine content, wheat pangenome resources facilitated analysis of candidate genes in this region of the genome. These findings can be used to select appropriate strategies for lysine biofortification and free asparagine reduction in wheat breeding programmes. 

KeywordsWheat; Quantitative trait loci; Mapping population; Asparagine; Acrylamide; Food safety; Processing contaminants; Crop composition; Grain quality; Lysine
Year of Publication2023
JournalThe Plant Genome
Journal citation16 (4), p. e20335
Digital Object Identifier (DOI)https://doi.org/10.1002/tpg2.20335
Web address (URL)https://acsess.onlinelibrary.wiley.com/doi/10.1002/tpg2.20335
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCrop management strategies for low asparagine grains to limit acrylamide-forming potential
Designing Future Wheat (DFW) [ISPG]
Field assessment of ultra-low asparagine, low acrylamide, gene edited wheat
Publisher's version
Accepted author manuscript
Supplemental file
Output statusPublished
Publication dates
Online03 May 2023
Publication process dates
Accepted13 Mar 2023
PublisherWiley
ISSN1940-3372

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