Generic workflow of a highly effective and easy anther culture method for both japonica and indica rice

A - Papers appearing in refereed journals

Guo, G., Liu, S., Zhang, S., Yang, L., Zong, Y., Halford, N. G., He, T., Gao, R., Guo, Z., Zhou, L., Liu, C., Wu, S. and Chen, Z. 2024. Generic workflow of a highly effective and easy anther culture method for both japonica and indica rice. Plants - Basel. 13 (17), p. 2531. https://doi.org/10.3390/plants13172531

AuthorsGuo, G., Liu, S., Zhang, S., Yang, L., Zong, Y., Halford, N. G., He, T., Gao, R., Guo, Z., Zhou, L., Liu, C., Wu, S. and Chen, Z.
Abstract

As one of the most important staple crops in the world, rice plays a pivotal role in world food se-curity. The creation of doubled haploids based on anther culture is an important technology for rice breeding. However, at present, rice anther culture technology still faces many problems, such as genotype dependency, especially genotypes of indica rice. In this study, fifteen rice genotypes, in-cluding twelve japonica rice genotypes and three indica rice genotypes, were randomly selected and used to study anther culture by using a modified M8 medium. The results showed that the total callus induction rates of these different rice genotypes ranged from 0.81 to 13.95%, with an average of 6.64%, while the callus induction rates calculated for the top ten highest callus inductions for each rice genotype ranged from 2.75 to 17.00%, with an average of 10.56%. There were varying gaps between the total callus induction rates and the callus induction rates in these different rice genotypes. The fact that the gaps for some rice genotypes were relatively large indicated that standard tiller or anther collection was not applicable to all rice genotypes and that there was still a lot of room for im-provement in the callus induction rate of some rice genotypes through optimization of the sampling method. The plantlet regeneration rates ranged from 12.55 to 456.54%, with an average of 200.10%. Although there were many albinos from anther culture for some rice genotypes, these would still meet the requirement if the rice genotypes had higher callus induction rates or regeneration rates. The percentages of seed setting of regenerated green seedlings ranged from 14% to 84%, with an average of 48.73%. Genetic diversity analysis showed that the genetic background of these different rice genotypes was representative, and the phylogenetic tree and Principal Component Analysis (PCA) divided them into indica and japonica types. Therefore, in this study, an anther culture method suitable for both indica and japonica rice genotypes was established, which could improve doubled haploid breeding in rice.

KeywordsOryza sativa L; Microspore; Callus induction; Plantlet regeneration; Ploidy
Year of Publication2024
JournalPlants - Basel
Journal citation13 (17), p. 2531
Digital Object Identifier (DOI)https://doi.org/10.3390/plants13172531
Web address (URL)https://www.mdpi.com/2223-7747/13/17/2531
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Shanghai Agricultural Science and Technology Innovation Program
Funder project or codeDesigning Future Wheat (DFW) [ISPG]
Publisher's version
Output statusPublished
Publication dates
Online09 Sep 2024
Publication process dates
Accepted05 Sep 2024
PublisherMDPI
ISSN2223-7747

Permalink - https://repository.rothamsted.ac.uk/item/991q5/generic-workflow-of-a-highly-effective-and-easy-anther-culture-method-for-both-japonica-and-indica-rice

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