Construction of a network describing asparagine metabolism in plants and its application to the identification of genes affecting asparagine metabolism in wheat under drought and nutritional stress

A - Papers appearing in refereed journals

Curtis, T. Y., Bo, V., Tucker A. and Halford, N. G. 2018. Construction of a network describing asparagine metabolism in plants and its application to the identification of genes affecting asparagine metabolism in wheat under drought and nutritional stress. Food and Energy Security. 7 (1), p. e00126.

AuthorsCurtis, T. Y., Bo, V., Tucker A. and Halford, N. G.
Abstract

A detailed network describing asparagine metabolism in plants was constructed using published data from Arabidopsis (Arabidopsis thaliana) maize (Zea mays), wheat (Triticum aestivum), pea (Pisum sativum), soybean (Glycine max), lupin (Lupus albus) and other species, including animals. Asparagine synthesis and degradation is a major part of amino acid and nitrogen metabolism in plants. The complexity of its metabolism, including limiting and regulatory factors, was represented in a logical sequence in a pathway diagram built using yED graph editor software. The network was used with the Unique Network Identification Pipeline in the analysis of data from 18 publicly-available transcriptomic data studies. This identified links between genes involved in asparagine metabolism in wheat roots under drought stress, wheat leaves under drought stress, and wheat leaves under conditions of sulphur and nitrogen deficiency. The network represents a powerful aid for interpreting the interactions not only between the genes in the pathway but also between enzymes, metabolites and smaller molecules. It provides a concise, clear understanding of the complexity of asparagine metabolism that could aid the interpretation of data relating to wider amino acid metabolism and other metabolic processes.

KeywordsAsparagine metabolism; Asparagine synthetase; Glutamine synthetase; Systems approaches; Stress responses; Biochemistry; Composition; Food Safety; Genetics
Year of Publication2018
JournalFood and Energy Security
Journal citation7 (1), p. e00126
Digital Object Identifier (DOI)doi:10.1002/fes3.126
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeGenetic improvement of wheat to reduce the potential for acrylamide formation during processing.
Designing Future Wheat (DFW) [ISPG]
BSRC-BB/I020918/1
DFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience
Accepted author manuscript
Output statusE-publication ahead of print
Publication dates
Online19 Jan 2018
Publication process dates
Accepted07 Jan 2018
PublisherWiley
Copyright licenseCC BY
ISSN2048-3694

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