Designing Future Wheat (DFW) [ISPG]

Repository project

Project dates01 Apr 2017 to end of 31 Mar 2022
Researchers
Principal InvestigatorMalcolm Hawkesford
FunderBiotechnology and Biological Sciences Research Council
Project numberI0200
DepartmentRothamsted Research
Research fish codeBB/P016855/1
Grant IDBB/P016855/1 [Do not use in Researchfish]

Outputs

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Membrane-permeable trehalose 6-phosphate precursor spray increases wheat yields in field trials

A - Papers appearing in refereed journals
Griffiths, C. A., Xue, X., Miret-Barrio, J., Salvagiotti, F., Acevedo-Siaca, L. G., Gimeno, J., Reynolds, M. P., Hassall, K., Halsey, K., Puranik, S., Oszvald, M., Kurup, S., Davis, B. G. and Paul, M. J. 2025. Membrane-permeable trehalose 6-phosphate precursor spray increases wheat yields in field trials. Nature Biotechnology. https://doi.org/10.1038/s41587-025-02611-1

Improved Extraction Methods to Isolate High Molecular Weight DNA From Magnaporthaceae and Other Grass Root Fungi for Long-Read Whole Genome Sequencing

A - Papers appearing in refereed journals
Grey, M. J., Freeman, J., Rudd, J. J., Irish, N., Canning, G., Chancellor, T., Palma-Guerrero, J., Hill, R., Hall, N., Hammond-Kosack, K. E. and McMullan, M. 2025. Improved Extraction Methods to Isolate High Molecular Weight DNA From Magnaporthaceae and Other Grass Root Fungi for Long-Read Whole Genome Sequencing. Bio-protocol. 15 (6), p. e5245. https://doi.org/10.21769/BioProtoc.5245

GIBBERELLIN 3-OXIDASE genes regulate height and grain size in bread wheat

A - Papers appearing in refereed journals
Phillips, A. L., Huttly, A. K., Alarcon-Reverte, R., Clark, S. J., Jaworek, P., Tarkowska, D., Sokolowska, P., Steele, D., Riche, A. B., Hawkesford, M. J., Thomas, S., Hedden, P. and Pearce, S. 2025. GIBBERELLIN 3-OXIDASE genes regulate height and grain size in bread wheat. Journal of Experimental Botany. p. efa151. https://doi.org/10.1093/jxb/eraf151

Ion homeostasis and coordinated salt tolerance mechanisms in a barley (Hordeum vulgare L.)doubled haploid line

A - Papers appearing in refereed journals
Xu, H., Chen, H., Halford, N. G., Xu, R., He, T., Yang, B., Zhou, L., Guo, H. and Liu, C. 2025. Ion homeostasis and coordinated salt tolerance mechanisms in a barley (Hordeum vulgare L.)doubled haploid line. BMC Plant Biology. 25, p. 52. https://doi.org/10.1186/s12870-024-06033-0

Evolutionary genomics reveals variation in structure and genetic content implicated in virulence and lifestyle in the genus Gaeumannomyces

A - Papers appearing in refereed journals
Hammond-Kosack, K. E., Hill, R., Grey, M., Fedi, M. O., Smith, D. P., Ward, S. J., Canning, G., Irish, N., Smith, J., Mcmillan, V. E., Hammond, J., Osborne, S., Chancellor, T., Swarbreck, D., Hall, N., Palma-Guerrero, J. and McMullan, M. 2025. Evolutionary genomics reveals variation in structure and genetic content implicated in virulence and lifestyle in the genus Gaeumannomyces. BMC Genomics. 26, p. 239. https://doi.org/10.1186/s12864-025-11432-0

Hyperspectral imaging for phenotyping plant drought stress and nitrogen interactions using multivariate modeling and machine learning techniques in wheat

A - Papers appearing in refereed journals
Okyere, F., Cudjoe, D., Virlet, N., Castle, M., Riche, A. B., Greche, L., Mohareb, F., Simms, D., Mhada, M. and Hawkesford, M. J. 2024. Hyperspectral imaging for phenotyping plant drought stress and nitrogen interactions using multivariate modeling and machine learning techniques in wheat. Remote Sensing. 16 (18), p. 3446. https://doi.org/10.3390/rs16183446

Generic workflow of a highly effective and easy anther culture method for both japonica and indica rice

A - Papers appearing in refereed journals
Guo, G., Liu, S., Zhang, S., Yang, L., Zong, Y., Halford, N. G., He, T., Gao, R., Guo, Z., Zhou, L., Liu, C., Wu, S. and Chen, Z. 2024. Generic workflow of a highly effective and easy anther culture method for both japonica and indica rice. Plants - Basel. 13 (17), p. 2531. https://doi.org/10.3390/plants13172531

Harnessing landrace diversity empowers wheat breeding

A - Papers appearing in refereed journals
Cheng, S., Feng, C., Wingen, L. U., Cheng, H., Riche, A. B., Jiang, M., Leverington-Waite, M., Huang, Z., Collier, S., Orford, S., Wang, X., Awal, R., Barker, G., O’Hara, T., Lister, C., Siluveru, A., Quiroz-Chávez, J., Ramírez-González, R. H., Bryant, R., Berry, S., Bansal, U., Bariana, H. S., Bennett, M. J., Bicego, B., Bilham, L., Brown, J. K. M., Burridge, A., Burt, C., Buurman, M., Castle, M., Chartrain, L., Chen, B., Denbel, W., Elkot, A. F., Fenwick, P., Feuerhelm, D., Foulkes, J., Gaju, O., Gauley, A., Gaurav, K., Hafeez, A. N., Han, R., Horler, R., Hou, J., Iqbal, M. S., Kerton, M., Kondic-Spica, A., Kowalski, A., Lage, J., Li, X., Liu, H., Liu, S., Lovegrove, A., Ma, L., Mumford, C., Parmar, S., Philp, C., Playford, D., Przewieslik-Allen, A. M., Sarfraz, Z., Schafer, D., Shewry, P. R., Shi, Y., Slafer, G., Song, B., Song, B., Steele, D., Steuernagel, B., Tailby, P., Tyrrell, S., Waheed, A., Wamalwa, M. N., Wang, X., Wei, Y., Winfield, M., Wu, S., Wu, Y., Wulff, B. B. H., Xian, W., Xu, Y., Xu, Y., Yuan, Q., Zhang, X., Edwards, K. J., Dixon, L., Nicholson, P., Chayut, N., Hawkesford, M. J., Uauy, C., Sanders, D., Huang, S. and Griffiths, S. 2024. Harnessing landrace diversity empowers wheat breeding. Nature. https://doi.org/10.1038/s41586-024-07682-9

Stacked mutations in wheat homologues of rice SEMI-DWARF1 confer a novel semi-dwarf phenotype

A - Papers appearing in refereed journals
Ndreca, B., Huttly, A. K., Bibi, S., Bayon, C., Lund, G., Ham, J., Alarcon-Reverte, R., Addy, J., Tarkowská, D., Pearce, S., Hedden, P., Thomas, S. G. and Phillips, A. L. 2024. Stacked mutations in wheat homologues of rice SEMI-DWARF1 confer a novel semi-dwarf phenotype. BMC Plant Biology. 24, p. 384. https://doi.org/10.1186/s12870-024-05098-1

The trichothecene mycotoxin deoxynivalenol facilitates cell-to-cell invasion during wheat-tissue colonization by Fusarium graminearum

A - Papers appearing in refereed journals
Armer, V., Urban, M., Ashfield, T., Deeks, M. J. and Hammond-Kosack, K. E. 2024. The trichothecene mycotoxin deoxynivalenol facilitates cell-to-cell invasion during wheat-tissue colonization by Fusarium graminearum. Molecular Plant Pathology. 25 (6), p. e13485. https://doi.org/10.1111/mpp.13485

Identification of universal grass genes and estimates of their monocot-/ commelinid-/ grass-specificity

A - Papers appearing in refereed journals
Mitchell, R. A. C. 2024. Identification of universal grass genes and estimates of their monocot-/ commelinid-/ grass-specificity. Bioinformatics Advances. p. vbaf079. https://doi.org/10.1093/bioadv/vbaf079

Unravelling the impact of soil types on zinc, iron, and selenium concentrations in grains and straw of wheat/Amblyopyrum muticum and wheat/Triticum urartu doubled haploid lines

A - Papers appearing in refereed journals
Guwela, V. F., Broadley, M., Hawkesford, M. J., Maliro, M. F. A., Bokosi, J., Banda, M., Grewal, S., Wilson, L. and King, J. 2024. Unravelling the impact of soil types on zinc, iron, and selenium concentrations in grains and straw of wheat/Amblyopyrum muticum and wheat/Triticum urartu doubled haploid lines. Frontiers in Agronomy. 6, p. 1305034. https://doi.org/10.3389/fagro.2024.1305034

Identification and functional characterisation of a locus for target site integration in Fusarium graminearum

A - Papers appearing in refereed journals
Darino, M., Urban, M., Kaur, N., Wood, A. K. M., Smith, D., Grimwade-Mann, M., Beacham, A. and Hammond-Kosack, K. E. 2024. Identification and functional characterisation of a locus for target site integration in Fusarium graminearum. Fungal Biology and Biotechnology. 11 (2), pp. 1-21. https://doi.org/10.1186/s40694-024-00171-8

The effects of soil compaction on wheat seedling root growth are specific to soil texture and soil moisture status

A - Papers appearing in refereed journals
Yu, C., Mawodza, T., Atkinson, B. S., Atkinson, J. A., Sturrock, C. J., Whalley, W. R., Hawkesford, M. J., Cooper, H., Zhang, X., Zhou, H. and Mooney, S. J. 2024. The effects of soil compaction on wheat seedling root growth are specific to soil texture and soil moisture status. Rhizosphere. 29, p. 100838. https://doi.org/10.1016/j.rhisph.2023.100838

universal_grass_peps

N - Datasets
Mitchell, R. A. C. 2024. universal_grass_peps. Rothamsted Research. https://doi.org/10.23637/rothamsted.98ywz

Root phenotyping and root water uptake calculation using soil water contents measured in a winter wheat field

A - Papers appearing in refereed journals
Huang, Z., Zhang, X., Ashton, R. W., Hawkesford, M. J. and Whalley, W. R. 2023. Root phenotyping and root water uptake calculation using soil water contents measured in a winter wheat field. Agricultural Water Management. 290, p. 108607. https://doi.org/10.1016/j.agwat.2023.108607

Soil structure has a greater effect on the rooting of wheat (Triticum aestivum L.) than nitrogen fertilisation rate or genotype.

A - Papers appearing in refereed journals
Mawodza, T., Zhou, H., Atkinson, B. S., Atkinson, J. A., Sturrock, C. J., Riche, A. B., Whalley, W. R., Hawkesford, M. J., Cooper, H. V. and Mooney, S. J. 2023. Soil structure has a greater effect on the rooting of wheat (Triticum aestivum L.) than nitrogen fertilisation rate or genotype. Rhizosphere. 27 (Sept), p. 100770. https://doi.org/10.1016/j.rhisph.2023.100770

Comparison of the impact of two key fungal signalling pathways on Zymoseptoria tritici infection reveals divergent contribution to invasive growth through distinct regulation of infection-associated genes

A - Papers appearing in refereed journals
Child, H. T., Deeks, M. J., Rudd, J. J. and Bates, S. 2023. Comparison of the impact of two key fungal signalling pathways on Zymoseptoria tritici infection reveals divergent contribution to invasive growth through distinct regulation of infection-associated genes. Molecular Plant Pathology. 24 (10), pp. 1220-1237. https://doi.org/10.1111/mpp.13365

AgMIP-Wheat multi-model simulations on climate change impact and adaptation for global wheat, SDATA-20-01059

A - Papers appearing in refereed journals
Liu, B., Martre, P., Ewert, F., Webber, H., Waha, K., Thorburn, P. J., Ruane, A. C., Aggarwal, P. K., Ahmed, M., Balkovic, J., Basso, B., Biernath, C., Bindi, M., Cammarano, D., Cao, W., Challinor, A. J., De Sanctis, G., Dumont, B., Espadafor, M., Rezaei, E. E., Fereres, E., Ferrise,. R., Garcia-Vila, M., Gao, Y., Horan, H., Hoogenboom, G., Izaurralde, R. C., Jabloun, M., Jones, C. D., Kassie, B. T., Kersebaum, K. C., Klein, C., Koehler ,A.-K., Maiorano, A., Minoli. S., San Martin, M. M., Muller, C., Kumar, S. N., Nendel, C., O’Leary, G. J., Olesen, J.E ., Palosuo, T., Porter, J.R., Priesack, E., Ripoche, D., Rotter, R. P., Semenov, M. A., Stockle, C., Stratonovitch, P., Streck, T., Supit, I., Tao, F., Van der Velde, M., Wang, E., Wolf, J., Xiao, L., Zhang, Z., Zhao, Z., Zhu, Y. and Asseng, S. 2023. AgMIP-Wheat multi-model simulations on climate change impact and adaptation for global wheat, SDATA-20-01059. Open Data Journal for Agricultural Research (ODjAR). 9, pp. 10-25. https://doi.org/10.18174/odjar.v9i0.18092

Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana

A - Papers appearing in refereed journals
Morales-Herrera, S., Jourquin, J., Coppe, F., Lopez-Galvis, L., De Smet, T., Safi, A., Njo, M., Griffiths, C. A., Sidda, J. D., Mccullagh, J. S. O., Xue, X., Davis, B. G., Van der Eycken, J., Paul, M. J., Van Dijck, P. and Beeckman, T. 2023. Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana. Proceedings of the National Academy of Sciences. 120 (40), p. e2302996120. https://doi.org/10.1073/pnas.2302996120

Analysis of mixed linkage β-glucan content and structure in different wheat flour milling fractions

A - Papers appearing in refereed journals
Prins, A., Shewry, P. R. and Lovegrove, A. 2023. Analysis of mixed linkage β-glucan content and structure in different wheat flour milling fractions. Journal of Cereal Science. 113, p. 103753. https://doi.org/10.1016/j.jcs.2023.103753

Delayed development of basal spikelets in wheat explains their increased floret abortion and rudimentary nature

A - Papers appearing in refereed journals
Backhaus, A. E., Griffiths, C. A., Vergara-Cruces, A., Simmonds, J., Lee, R., Morris, R. J. and Uauy, C. 2023. Delayed development of basal spikelets in wheat explains their increased floret abortion and rudimentary nature. Journal of Experimental Botany. 74 (17), pp. 5088-5103. https://doi.org/10.1093/jxb/erad233

Aggregation of chromosome axis proteins on the chromatin and in the nucleoplasm of Brassica oleracea meiocytes

A - Papers appearing in refereed journals
Shan, W. and Lambing, C. 2023. Aggregation of chromosome axis proteins on the chromatin and in the nucleoplasm of Brassica oleracea meiocytes. Crop Design. 2 (2), p. 100038. https://doi.org/10.1016/j.cropd.2023.100038

Real-Time Quantitative PCR - Primer Design, Reference Gene Selection, Calculations and Statistics

A - Papers appearing in refereed journals
Chen, Z., Halford, N. G. and Liu, C. 2023. Real-Time Quantitative PCR - Primer Design, Reference Gene Selection, Calculations and Statistics. Metabolites. 13 (7), p. 806. https://doi.org/10.3390/metabo13070806

PHOSPHORUS-STARVATION TOLERANCE 1 (OsPSTOL1) is prevalent in upland rice and enhances root growth and hastens low phosphate signaling in wheat

A - Papers appearing in refereed journals
Kettenburg, A. T., Lopez, M. A., Yogendra, K., Prior, M. J., Rose, T., Bimson, S., Heuer, S., Roy, S. J. and Bailey-Serres, J. 2023. PHOSPHORUS-STARVATION TOLERANCE 1 (OsPSTOL1) is prevalent in upland rice and enhances root growth and hastens low phosphate signaling in wheat. Plant, Cell and Environment. https://doi.org/10.1111/pce.14588

A modified Agrobacterium-mediated transformation for two oomycete pathogens

A - Papers appearing in refereed journals
Wang, L., Zhao, F., Liu, H., Chen, H., Zhang, F., Li, S., Sun, T., Nekrasov, V., Huang, S. and Dong, S. 2023. A modified Agrobacterium-mediated transformation for two oomycete pathogens. PLOS Pathogens. 19 (4), p. e1011346. https://doi.org/10.1371/journal.ppat.1011346

Towards stability of food production and farm income in a variable climate

A - Papers appearing in refereed journals
Harkness, C., Areal, F. J., Semenov, M. A., Senapati, N., Shield, I. F. and Bishop, J. 2023. Towards stability of food production and farm income in a variable climate. Ecological Economics. 204 (Part A), p. 107676. https://doi.org/10.1016/j.ecolecon.2022.107676

Field assessment of genome edited, low asparagine wheat: Europe's first CRISPR wheat field trial.

A - Papers appearing in refereed journals
Raffan, S., Oddy, J., Mead, A., Barker, G., Curtis, T. Y., Usher, S. L., Burt, C. and Halford, N. G. 2023. Field assessment of genome edited, low asparagine wheat: Europe's first CRISPR wheat field trial. Plant Biotechnology Journal. 21 (6), pp. 1097-1099. https://doi.org/10.1111/pbi.14026

Genetic diversity and population structure analysis of barley landraces from Shanghai region using genotyping-by-sequencing

A - Papers appearing in refereed journals
Li, L., Halford, N. G., Wang, H., Zong, Y., Guo, Z., Lu, R., Liu, C. and Chen, Z. 2023. Genetic diversity and population structure analysis of barley landraces from Shanghai region using genotyping-by-sequencing. Phyton. 92 (4), pp. 1275-1287. https://doi.org/10.32604/phyton.2023.026946

Accounting for environmental variation in the free asparagine content of wheat grain

A - Papers appearing in refereed journals
Oddy, J., Elmore, S. and Halford, N. G. 2023. Accounting for environmental variation in the free asparagine content of wheat grain. Journal of Food Composition and Analysis - JFCA. 120, p. 105333. https://doi.org/10.1016/j.jfca.2023.105333

Reducing dietary acrylamide exposure from wheat products through crop management and imaging

A - Papers appearing in refereed journals
Oddy, J., Addy, J., Mead, A., Hall, C., Mackay, C., Ashfield, T., Mcdiarmid, F., Curtis, T. Y., Raffan, S., Wilkinson, M. D., Elmore, J. S., Cryer, N., Moreira de Almeida, I. and Halford, N. G. 2023. Reducing dietary acrylamide exposure from wheat products through crop management and imaging. Journal of Agricultural and Food Chemistry - JAFC. 71 (7), pp. 3403-3413. https://doi.org/10.1021/acs.jafc.2c07208

A framework for community curation of interspecies interactions literature

A - Papers appearing in refereed journals
Cuzick, A., Seager, J., Wood, V., Urban, M., Rutherford, K. and Hammond-Kosack, K. E. 2023. A framework for community curation of interspecies interactions literature. eLife. 12, p. e84658. https://doi.org/10.7554/eLife.84658

Genetic control of grain amino acid composition in a UK soft wheat mapping population

A - Papers appearing in refereed journals
Oddy, J., Chhetry, M., Awal, R., Addy, J., Wilkinson, M. D., Smith, D., King, R., Hall, C., Testa, R., Murray, E., Raffan, S., Curtis, T. Y., Wingen, L., Griffiths, S., Berry, S., Elmore, S. J., Cryer, N., Moreira de Almeida, I. and Halford, N. G. 2023. Genetic control of grain amino acid composition in a UK soft wheat mapping population. The Plant Genome. 16 (4), p. e20335. https://doi.org/10.1002/tpg2.20335

Meiotic chromosome organization and its role in recombination and cancer

A - Papers appearing in refereed journals
Morgan, C., Nayak, A., Hosoya, N., Smith, G. R. and Lambing, C. 2023. Meiotic chromosome organization and its role in recombination and cancer. Current topics in developmental biology. 151 (chpt 5), pp. 91-126. https://doi.org/10.1016/bs.ctdb.2022.04.008

A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis

A - Papers appearing in refereed journals
Piovesana, M., Wood, A. K. M., Smith, D. P., Deery, M. J., Bayliss, R., Carrera, E., Napier, J. A., Kurup, S. and Matthes, M. C. 2023. A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis. Journal of Experimental Botany. 74 (10), pp. 3104-3121. https://doi.org/10.1093/jxb/erad080

Time series canopy phenotyping enables the identification of genetic variants controlling dynamic phenotypes in soybean

A - Papers appearing in refereed journals
Li, D., Bai, D., Tian, Y., Li, Y.‐H., Zhao, C., Wang, Q., Guo, S., Luan, X., Wang, R., Yang, J., Hawkesford, M. J., Schnable, J. C., Jin, X. and Qiu, L. 2022. Time series canopy phenotyping enables the identification of genetic variants controlling dynamic phenotypes in soybean. Journal of Integrative Plant Biology. https://doi.org/10.1111/jipb.13380

Improving crop yield potential Underlying biological processes and future prospects

A - Papers appearing in refereed journals
Burgess, A. J., Masclaux-Daubresse, C., Strittmatter, G., Weber, A. P. M., Taylor, S. H., Harbinson, J., Yin, X., Freeman, S. E., Paul, M. J., Westhoff, P., Loreto, F., Ceriotti, A., Saltenis, V. L. R., Pribil, M., Nacry, P., Scharff, L. B., Jensen, P. E., Muller, B., Cohan, J., Foulkes, J., Rogowsky, P., Debaeke, P., Meyer, C., Nelissen, H., Inze, D., Lankhorst, R. K., Parry, M. A. J., Murchie, E. H. and Baekelandt, A. 2022. Improving crop yield potential Underlying biological processes and future prospects. Food and Energy Security. p. e435. https://doi.org/10.1002/fes3.435

Vegetable Crop Improvement Through CRISPR Technology for Food Security

B - Book chapters etc edited externally
Bhattacharya, J., Chaitanya, A. K., Hegde, N., Singh, S., Kour, A. and Nitnavare, R. 2022. Vegetable Crop Improvement Through CRISPR Technology for Food Security. in: Wani, S. H and Hensel, G. (ed.) Genome Editing - Current Technology Advances and Applications for Crop Improvement Switzerland Springer. pp. 27-53

Distinct roles for different autophagy-associated genes in the virulence of the fungal wheat pathogen Zymoseptoria tritici

A - Papers appearing in refereed journals
Child, H. T., Deeks, M. J., Haynes, K., Rudd, J. J. and Bates, S. 2022. Distinct roles for different autophagy-associated genes in the virulence of the fungal wheat pathogen Zymoseptoria tritici. Fungal Genetics and Biology. 163, p. 103748. https://doi.org/10.1016/j.fgb.2022.103748

Apoplastic and vascular defences

A - Papers appearing in refereed journals
Darino, M., Kanyuka, K. and Hammond-Kosack, K. E. 2022. Apoplastic and vascular defences. Essays in Biochemistry. 66 (5), pp. 595-605. https://doi.org/10.1042/EBC20220159