Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana

A - Papers appearing in refereed journals

Morales-Herrera, S., Jourquin, J., Coppe, F., Lopez-Galvis, L., De Smet, T., Safi, A., Njo, M., Griffiths, C. A., Sidda, J. D., Mccullagh, J. S. O., Xue, X., Davis, B. G., Van der Eycken, J., Paul, M. J., Van Dijck, P. and Beeckman, T. 2023. Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana. Proceedings of the National Academy of Sciences. 120 (40), p. e2302996120. https://doi.org/10.1073/pnas.2302996120

AuthorsMorales-Herrera, S., Jourquin, J., Coppe, F., Lopez-Galvis, L., De Smet, T., Safi, A., Njo, M., Griffiths, C. A., Sidda, J. D., Mccullagh, J. S. O., Xue, X., Davis, B. G., Van der Eycken, J., Paul, M. J., Van Dijck, P. and Beeckman, T.
Abstract

Plant roots explore the soil for water and nutrients, thereby determining plant fitness and agricultural yield, as well as determining ground substructure, water levels, and global carbon sequestration. The colonization of the soil requires investment of carbon and energy, but how sugar and energy signaling are integrated with root branching is unknown. Here, we show through combined genetic and chemical modulation of signaling pathways that the sugar small-molecule signal, trehalose-6-phosphate (T6P) regulates root branching through master kinases SNF1-related kinase-1 (SnRK1) and Target of Rapamycin (TOR) and with the involvement of the plant hormone auxin. Increase of T6P levels both via genetic targeting in lateral root (LR) founder cells and through light-activated release of the presignaling T6P-precursor reveals that T6P increases root branching through coordinated inhibition of SnRK1 and activation of TOR. Auxin, the master regulator of LR formation, impacts this T6P function by transcriptionally down-regulating the T6P-degrader trehalose phosphate phosphatase B in LR cells. Our results reveal a regulatory energy-balance network for LR formation that links the ‘sugar signal’ T6P to both SnRK1 and TOR downstream of auxin.

Year of Publication2023
JournalProceedings of the National Academy of Sciences
Journal citation120 (40), p. e2302996120
Digital Object Identifier (DOI)https://doi.org/10.1073/pnas.2302996120
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeUnderstanding a mutant that disregulates trehalose 6-phosphate action in plants
Designing Future Wheat (DFW) [ISPG]
Publisher's version
Supplemental file
Supplemental file
Output statusPublished
Publication dates
Online25 Sep 2023
Publication process dates
Accepted08 Aug 2023
PublisherNational Academy of Sciences of the United States of America
ISSN0027-8424

Permalink - https://repository.rothamsted.ac.uk/item/98y24/trehalose-6-phosphate-signaling-regulates-lateral-root-formation-in-arabidopsis-thaliana

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