Gene-based mapping of trehalose biosynthetic pathway genes reveals association with source- and sink-related yield traits in a spring wheat panel

A - Papers appearing in refereed journals

Lyra, D. H., Griffiths, C. A., Watson, A., Joynson, R., Molero, G., Igna, A. A., Hassani-Pak, K., Reynolds, M. P., Hall, A. and Paul, M. J. 2021. Gene-based mapping of trehalose biosynthetic pathway genes reveals association with source- and sink-related yield traits in a spring wheat panel. Food and Energy Security. p. e292. https://doi.org/10.1002/fes3.292

AuthorsLyra, D. H., Griffiths, C. A., Watson, A., Joynson, R., Molero, G., Igna, A. A., Hassani-Pak, K., Reynolds, M. P., Hall, A. and Paul, M. J.
Abstract

Trehalose 6-phosphate (T6P) signalling regulates carbon use and allocation and is a target to improve crop yields. However, the specific contributions of trehalose phosphate synthase (TPS) and trehalose phosphate phosphatase (TPP) genes to source- and sink-related traits remain largely unknown. We used enrichment-capture sequencing on TPS and TPP genes to estimate and partition the genetic variation of yield-related traits in a spring wheat (Triticum aestivum) breeding panel specifically built to capture the diversity across the 75,000 CIMMYT wheat cultivar collection. Twelve phenotypes were correlated to variation in TPS and TPP genes including plant height and biomass (source), spikelets per spike, spike growth, and grain filling traits (sink) which showed indications of both positive and negative gene selection. Individual genes explained proportions of heritability for biomass and grain-related traits. Three TPS1 homeologues were particularly significant for trait variation. Epistatic interactions were found within and between the TPS and TPP gene families for both plant height and grain-related traits. Gene-based prediction improved predictive ability for grain weight when gene effects were combined with the whole-genome markers. Our study has generated a wealth of information on natural variation of TPS and TPP genes related to yield potential which confirms the role for T6P in resource allocation and in affecting traits such as grain number and size confirming other studies which now opens up the possibility of harnessing natural genetic variation more widely to better understand the contribution of native genes to yield traits for incorporation into breeding programmes.

KeywordsExome-capture sequencing; Gene-based association analysis; Gene-based prediction; High Biomass Association Mapping Panel (HiBAP); Trehalose phosphate synthase (TPS); Trehalose phosphate phosphatase (TPP); Regional heritability mapping; Signature of selection
Year of Publication2021
JournalFood and Energy Security
Journal citationp. e292
Digital Object Identifier (DOI)https://doi.org/10.1002/fes3.292
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat - WP1 - Increased efficiency and sustainability
International Wheat Yield Partnership Grant (Transforming yield through source-sink optimisation) (RP10317-01)
Publisher's version
Output statusPublished
Publication dates
Online07 May 2021
Publication process dates
Accepted12 Apr 2021
PublisherWiley
ISSN2048-3694

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